NBP21519301

CD45RA Antibody (158-4D3), Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NBP21519301-Each-of-1 In Stock ₹ 44,833.44

NBP21519301 - Each of 1

₹ 44,833.44

In Stock

Quantity

1

Base Price: ₹ 44,833.44

GST (18%): ₹ 8,070.019

Total Price: ₹ 52,903.459

Antigen

CD45RA

Classification

Monoclonal

Concentration

0.2mg/mL

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml

Gene Accession No.

P08575

Gene Symbols

PTPRC

Immunogen

Stimulated human leukocytes were used as immunogen to generate the CD45RA antibody.

Quantity

0.1 mg

Primary or Secondary

Primary

Test Specificity

Recognizes a protein of 205kDa-220kDa, identified as CD45RA [Workshop V; Code CD45.38]. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Content And Storage

Store at 4C.

Isotype

IgG2a κ

Applications

Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone

158-4D3

Conjugate

Unconjugated

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Host Species

Mouse

Purification Method

Protein G purified

Regulatory Status

RUO

Gene ID (Entrez)

5788

Target Species

Human, Primate

Form

Purified

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Description

  • CD45RA Monoclonal specifically detects CD45RA in Human samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Compare Similar Items

Show Difference

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Novus Biologicals

NBP21519301

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Antigen:
CD45RA

Classification:
Monoclonal

Concentration:
0.2mg/mL

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml

Gene Accession No.:
P08575

Gene Symbols:
PTPRC

Immunogen:
Stimulated human leukocytes were used as immunogen to generate the CD45RA antibody.

Quantity:
0.1 mg

Primary or Secondary:
Primary

Test Specificity:
Recognizes a protein of 205kDa-220kDa, identified as CD45RA [Workshop V; Code CD45.38]. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Content And Storage:
Store at 4C.

Isotype:
IgG2a κ

Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone:
158-4D3

Conjugate:
Unconjugated

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Host Species:
Mouse

Purification Method:
Protein G purified

Regulatory Status:
RUO

Gene ID (Entrez):
5788

Target Species:
Human, Primate

Form:
Purified

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NBP21519601

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Antigen:
Mucin 5AC

Classification:
Monoclonal

Concentration:
0.2mg/mL

Dilution:
Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5ug/ml, Flow (Intracellular)

Gene Accession No.:
P98088

Gene Symbols:
MUC5AC

Immunogen:
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Quantity:
0.1 mg

Primary or Secondary:
Primary

Test Specificity:
This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

Content And Storage:
Store at 4C.

Isotype:
IgG1 κ

Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone:
45M1

Conjugate:
Unconjugated

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
gastric mucin, leB, lewis B blood group antigen, major airway glycoprotein, MUC5, mucin 5, subtypes A and C, tracheobronchial/gastric, mucin 5AC, oligomeric mucus/gel-forming, mucin 5AC, oligomeric mucus/gel-forming pseudogene, mucin-5 subtype AC, tracheobronchial, mucin-5AC, TBM, tracheobronchial mucin

Host Species:
Mouse

Purification Method:
Protein G purified

Regulatory Status:
RUO

Gene ID (Entrez):
4586

Target Species:
Human, Mouse, Rat, Porcine, Chicken, Feline, Primate, Rabbit, Bovine (Negative)

Form:
Purified

Img

Fischer Scientific

NBP21519901

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Antigen:
Renal Cell Carcinoma (gp200)

Classification:
Monoclonal

Concentration:
0.2mg/mL

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml

Gene Accession No.:
Q16790

Gene Symbols:
LY75

Immunogen:
Fresh, normal human renal cortical tissue homogenate was used as immunogen to generate the RCC antibody.

Quantity:
0.1 mg

Primary or Secondary:
Primary

Test Specificity:
Recognizes a glycoprotein of ∼200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Content And Storage:
Store at 4C.

Isotype:
IgG2b κ

Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone:
66.4.C2 (PN-15)

Conjugate:
Unconjugated

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
CD205LY-75, CLEC13BLy-75, C-type lectin domain family 13 member B, DEC-205CD205 antigen, GP200-MR6, lymphocyte antigen 75

Host Species:
Mouse

Purification Method:
Protein G purified

Regulatory Status:
RUO

Gene ID (Entrez):
4065

Target Species:
Human, Equine

Form:
Purified

Img

Fischer Scientific

NBP21519902

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Antigen:
Renal Cell Carcinoma (gp200)

Classification:
Monoclonal

Concentration:
0.2mg/mL

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml

Gene Accession No.:
Q16790

Gene Symbols:
LY75

Immunogen:
Fresh, normal human renal cortical tissue homogenate was used as immunogen to generate the RCC antibody.

Quantity:
0.2 mg

Primary or Secondary:
Primary

Test Specificity:
Recognizes a glycoprotein of ∼200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Content And Storage:
Store at 4C.

Isotype:
IgG2b κ

Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone:
66.4.C2 (PN-15)

Conjugate:
Unconjugated

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
CD205LY-75, CLEC13BLy-75, C-type lectin domain family 13 member B, DEC-205CD205 antigen, GP200-MR6, lymphocyte antigen 75

Host Species:
Mouse

Purification Method:
Protein G purified

Regulatory Status:
RUO

Gene ID (Entrez):
4065

Target Species:
Human, Equine

Form:
Purified