NBP232977B

Melan-A/MART-1 Mouse, Clone: SPM540, Novus Biologicals™

Manufacturer: Fischer Scientific

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Antigen

Melan-A/MART-1

Classification

Monoclonal

Conjugate

Unconjugated

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

Antigen LB39-AA, Antigen SK29-AA, Mart 1 Melan A, MART1MART-1, melan-A, melanoma antigen recognized by T-cells 1, Protein Melan-A

Host Species

Mouse

Purification Method

Protein A purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Content And Storage

Store at 4C.

Isotype

IgG2b κ

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone

SPM540

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Gene Accession No.

Q16655

Gene Symbols

MLANA

Immunogen

Recombinant hMART-1 protein

Quantity

0.2 mg

Research Discipline

Cytoskeleton Markers, Immunology

Gene ID (Entrez)

2315

Target Species

Human, Mouse, Rat

Form

Purified

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Description

  • Melan-A/MART-1 Monoclonal specifically detects Melan-A/MART-1 in Human, Mouse, Rat samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

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Fischer Scientific

NBP232977B

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Antigen:
Melan-A/MART-1

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
Antigen LB39-AA, Antigen SK29-AA, Mart 1 Melan A, MART1MART-1, melan-A, melanoma antigen recognized by T-cells 1, Protein Melan-A

Host Species:
Mouse

Purification Method:
Protein A purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Content And Storage:
Store at 4C.

Isotype:
IgG2b κ

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone:
SPM540

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Gene Accession No.:
Q16655

Gene Symbols:
MLANA

Immunogen:
Recombinant hMART-1 protein

Quantity:
0.2 mg

Research Discipline:
Cytoskeleton Markers, Immunology

Gene ID (Entrez):
2315

Target Species:
Human, Mouse, Rat

Form:
Purified

Img

Fischer Scientific

NBP232977F

--


Antigen:
Melan-A/MART-1

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
Antigen LB39-AA, Antigen SK29-AA, Mart 1 Melan A, MART1MART-1, melan-A, melanoma antigen recognized by T-cells 1, Protein Melan-A

Host Species:
Mouse

Purification Method:
Protein A purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Content And Storage:
Store at 4C.

Isotype:
IgG2b κ

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone:
SPM540

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Gene Accession No.:
Q16655

Gene Symbols:
MLANA

Immunogen:
Recombinant hMART-1 protein

Quantity:
0.02 mg

Research Discipline:
Cytoskeleton Markers, Immunology

Gene ID (Entrez):
2315

Target Species:
Human, Mouse, Rat

Form:
Purified

Img

Fischer Scientific

NBP232984A

--


Antigen:
MUC2

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
Intestinal mucin-2, MLP, MUC-2, mucin 2, intestinal/tracheal, mucin 2, oligomeric mucus/gel-forming, mucin-2, mucin-like protein, SMUC

Host Species:
Mouse

Purification Method:
Protein A purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium.MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

Content And Storage:
Store at 4C.

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone:
SPM296

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Gene Accession No.:
Q02817

Gene Symbols:
MUC2

Immunogen:
A synthetic peptide of 29 amino acids KYPTTTPISTTTMVTPTPTPTGTQTPTTT from MUC2 protein, coupled to KLH.

Quantity:
0.1 mg

Research Discipline:
__

Gene ID (Entrez):
4583

Target Species:
Human

Form:
Purified

Img

Fischer Scientific

NBP232984B

--


Antigen:
MUC2

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
Intestinal mucin-2, MLP, MUC-2, mucin 2, intestinal/tracheal, mucin 2, oligomeric mucus/gel-forming, mucin-2, mucin-like protein, SMUC

Host Species:
Mouse

Purification Method:
Protein A purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium.MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

Content And Storage:
Store at 4C.

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone:
SPM296

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Gene Accession No.:
Q02817

Gene Symbols:
MUC2

Immunogen:
A synthetic peptide of 29 amino acids KYPTTTPISTTTMVTPTPTPTGTQTPTTT from MUC2 protein, coupled to KLH.

Quantity:
0.2 mg

Research Discipline:
__

Gene ID (Entrez):
4583

Target Species:
Human

Form:
Purified