NBP24461301
Nucleolin Antibody (364-5 + NCL/902), Novus Biologicals™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NBP24461301-Each-of-1 | In Stock | ₹ 44,833.44 |
NBP24461301 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,833.44
GST (18%): ₹ 8,070.019
Total Price: ₹ 52,903.459
Antigen
Nucleolin
Classification
Monoclonal
Concentration
0.2mg/mL
Dilution
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Gene Accession No.
P19338
Gene Symbols
NCL
Immunogen
Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902)
Purification Method
Protein A or G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Content And Storage
Store at 4C.
Isotype
IgG
Applications
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone
364-5 + NCL/902
Conjugate
Unconjugated
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias
C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Host Species
Mouse
Molecular Weight of Antigen
76 kDa
Quantity
0.1 mg
Research Discipline
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Gene ID (Entrez)
4691
Target Species
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Form
Purified
Related Products
Description
- Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence Nucleolin Monoclonal specifically detects Nucleolin in Human, Bovine (Negative), Mouse (Negative), Rat (Negative) samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
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Antigen: Nucleolin
Classification: Monoclonal
Concentration: 0.2mg/mL
Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Gene Accession No.: P19338
Gene Symbols: NCL
Immunogen: Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902)
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Content And Storage: Store at 4C.
Isotype: IgG
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone: 364-5 + NCL/902
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Host Species: Mouse
Molecular Weight of Antigen: 76 kDa
Quantity: 0.1 mg
Research Discipline: Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Gene ID (Entrez): 4691
Target Species: Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Form: Purified
Antigen:
Nucleolin
Classification:
Monoclonal
Concentration:
0.2mg/mL
Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Gene Accession No.:
P19338
Gene Symbols:
NCL
Immunogen:
Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902)
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Content And Storage:
Store at 4C.
Isotype:
IgG
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone:
364-5 + NCL/902
Conjugate:
Unconjugated
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Host Species:
Mouse
Molecular Weight of Antigen:
76 kDa
Quantity:
0.1 mg
Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Gene ID (Entrez):
4691
Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Form:
Purified
Antigen: Nucleolin
Classification: Monoclonal
Concentration: 0.2mg/mL
Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Gene Accession No.: P19338
Gene Symbols: NCL
Immunogen: Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902)
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Content And Storage: Store at 4C.
Isotype: IgG
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone: 364-5 + NCL/902
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Host Species: Mouse
Molecular Weight of Antigen: 76 kDa
Quantity: 0.2 mg
Research Discipline: Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Gene ID (Entrez): 4691
Target Species: Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Form: Purified
Antigen:
Nucleolin
Classification:
Monoclonal
Concentration:
0.2mg/mL
Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Gene Accession No.:
P19338
Gene Symbols:
NCL
Immunogen:
Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902)
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Content And Storage:
Store at 4C.
Isotype:
IgG
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone:
364-5 + NCL/902
Conjugate:
Unconjugated
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Host Species:
Mouse
Molecular Weight of Antigen:
76 kDa
Quantity:
0.2 mg
Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Gene ID (Entrez):
4691
Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Form:
Purified
Antigen: VCAM-1/CD106
Classification: Monoclonal
Concentration: 0.2 mg/mL
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Gene Accession No.: P19320
Gene Symbols: VCAM1
Immunogen: Activated human umbilical vein endothelial cells (HUVEC)
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This MAb inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Applications: Flow Cytometry, SDS-Page, Immunofluorescence
Clone: B-K9
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1
Host Species: Mouse
Molecular Weight of Antigen: 110 kDa
Quantity: 0.02 mg
Research Discipline: Cancer, Mesenchymal Stem Cell Markers, Stem Cell Markers
Gene ID (Entrez): 7412
Target Species: Human
Form: Purified
Antigen:
VCAM-1/CD106
Classification:
Monoclonal
Concentration:
0.2 mg/mL
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Gene Accession No.:
P19320
Gene Symbols:
VCAM1
Immunogen:
Activated human umbilical vein endothelial cells (HUVEC)
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This MAb inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.
Content And Storage:
Store at 4C.
Isotype:
IgG1 κ
Applications:
Flow Cytometry, SDS-Page, Immunofluorescence
Clone:
B-K9
Conjugate:
Unconjugated
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1
Host Species:
Mouse
Molecular Weight of Antigen:
110 kDa
Quantity:
0.02 mg
Research Discipline:
Cancer, Mesenchymal Stem Cell Markers, Stem Cell Markers
Gene ID (Entrez):
7412
Target Species:
Human
Form:
Purified
Antigen: VCAM-1/CD106
Classification: Monoclonal
Concentration: 0.2 mg/mL
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Gene Accession No.: P19320
Gene Symbols: VCAM1
Immunogen: Activated human umbilical vein endothelial cells (HUVEC)
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This MAb inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Applications: Flow Cytometry, SDS-Page, Immunofluorescence
Clone: B-K9
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1
Host Species: Mouse
Molecular Weight of Antigen: 110 kDa
Quantity: 0.1 mg
Research Discipline: Cancer, Mesenchymal Stem Cell Markers, Stem Cell Markers
Gene ID (Entrez): 7412
Target Species: Human
Form: Purified
Antigen:
VCAM-1/CD106
Classification:
Monoclonal
Concentration:
0.2 mg/mL
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Gene Accession No.:
P19320
Gene Symbols:
VCAM1
Immunogen:
Activated human umbilical vein endothelial cells (HUVEC)
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This MAb inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.
Content And Storage:
Store at 4C.
Isotype:
IgG1 κ
Applications:
Flow Cytometry, SDS-Page, Immunofluorescence
Clone:
B-K9
Conjugate:
Unconjugated
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1
Host Species:
Mouse
Molecular Weight of Antigen:
110 kDa
Quantity:
0.1 mg
Research Discipline:
Cancer, Mesenchymal Stem Cell Markers, Stem Cell Markers
Gene ID (Entrez):
7412
Target Species:
Human
Form:
Purified