NBP24468800
Cyclin B1 Antibody (SPM619), Novus Biologicals™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NBP24468800-Each-of-1 | In Stock | ₹ 22,930.08 |
NBP24468800 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 22,930.08
GST (18%): ₹ 4,127.414
Total Price: ₹ 27,057.494
Antigen
Cyclin B1
Classification
Monoclonal
Concentration
0.2mg/mL
Dilution
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Gene Alias
CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1
Host Species
Mouse
Purification Method
Protein A or G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Content And Storage
Store at 4C.
Isotype
IgG1 κ
Applications
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone
SPM619
Conjugate
Unconjugated
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols
CCNB1
Immunogen
Recombinant human full-length CCNB1 protein
Quantity
0.02 mg
Research Discipline
Cancer, Cell Biology, Cell Cycle and Replication, Mitotic Regulators, Tumor Suppressors
Gene ID (Entrez)
891
Target Species
Human, Mouse
Form
Purified
Related Products
Description
- Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence Cyclin B1 Monoclonal specifically detects Cyclin B1 in Human, Mouse samples
- It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.
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Antigen: Cyclin B1
Classification: Monoclonal
Concentration: 0.2mg/mL
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Gene Alias: CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone: SPM619
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols: CCNB1
Immunogen: Recombinant human full-length CCNB1 protein
Quantity: 0.02 mg
Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Mitotic Regulators, Tumor Suppressors
Gene ID (Entrez): 891
Target Species: Human, Mouse
Form: Purified
Antigen:
Cyclin B1
Classification:
Monoclonal
Concentration:
0.2mg/mL
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Gene Alias:
CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Content And Storage:
Store at 4C.
Isotype:
IgG1 κ
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone:
SPM619
Conjugate:
Unconjugated
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols:
CCNB1
Immunogen:
Recombinant human full-length CCNB1 protein
Quantity:
0.02 mg
Research Discipline:
Cancer, Cell Biology, Cell Cycle and Replication, Mitotic Regulators, Tumor Suppressors
Gene ID (Entrez):
891
Target Species:
Human, Mouse
Form:
Purified
Antigen: Cyclin B1
Classification: Monoclonal
Concentration: 0.2mg/mL
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Gene Alias: CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone: SPM619
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols: CCNB1
Immunogen: Recombinant human full-length CCNB1 protein
Quantity: 0.1 mg
Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Mitotic Regulators, Tumor Suppressors
Gene ID (Entrez): 891
Target Species: Human, Mouse
Form: Purified
Antigen:
Cyclin B1
Classification:
Monoclonal
Concentration:
0.2mg/mL
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Gene Alias:
CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Content And Storage:
Store at 4C.
Isotype:
IgG1 κ
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone:
SPM619
Conjugate:
Unconjugated
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols:
CCNB1
Immunogen:
Recombinant human full-length CCNB1 protein
Quantity:
0.1 mg
Research Discipline:
Cancer, Cell Biology, Cell Cycle and Replication, Mitotic Regulators, Tumor Suppressors
Gene ID (Entrez):
891
Target Species:
Human, Mouse
Form:
Purified
Antigen: Cyclin B1
Classification: Monoclonal
Concentration: 0.2mg/mL
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Gene Alias: CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone: SPM619
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols: CCNB1
Immunogen: Recombinant human full-length CCNB1 protein
Quantity: 0.2 mg
Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Mitotic Regulators, Tumor Suppressors
Gene ID (Entrez): 891
Target Species: Human, Mouse
Form: Purified
Antigen:
Cyclin B1
Classification:
Monoclonal
Concentration:
0.2mg/mL
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Gene Alias:
CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Content And Storage:
Store at 4C.
Isotype:
IgG1 κ
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone:
SPM619
Conjugate:
Unconjugated
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols:
CCNB1
Immunogen:
Recombinant human full-length CCNB1 protein
Quantity:
0.2 mg
Research Discipline:
Cancer, Cell Biology, Cell Cycle and Replication, Mitotic Regulators, Tumor Suppressors
Gene ID (Entrez):
891
Target Species:
Human, Mouse
Form:
Purified
Antigen: gp96/HSP90B1/GRP94
Classification: Monoclonal
Concentration: 0.2mg/mL
Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Gene Alias: 94 kDa glucose-regulated protein, endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, GP96ECGP, GRP-94, GRP94gp96 homolog, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, TRA1endoplasmin, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen-1 (gp96)
Host Species: Rat
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
Content And Storage: Store at 4C.
Isotype: IgG2a κ
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone: 9G10.F8.2
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols: HSP90B1
Immunogen: Purified glucose regulated protein 94 (grp94) from chicken oviducts
Quantity: 0.02 mg
Research Discipline: Cancer, Cellular Markers, ER Markers, Golgi Apparatus Markers, Hypoxia, Membrane Trafficking and Chaperones
Gene ID (Entrez): 7184
Target Species: Human, Mouse, Rat, Porcine, Canine, Chicken, Equine, Guinea Pig, Hamster, Monkey, Primate, Rabbit, Sheep, Xenopus species
Form: Purified
Antigen:
gp96/HSP90B1/GRP94
Classification:
Monoclonal
Concentration:
0.2mg/mL
Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Gene Alias:
94 kDa glucose-regulated protein, endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, GP96ECGP, GRP-94, GRP94gp96 homolog, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, TRA1endoplasmin, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen-1 (gp96)
Host Species:
Rat
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
Content And Storage:
Store at 4C.
Isotype:
IgG2a κ
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Clone:
9G10.F8.2
Conjugate:
Unconjugated
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols:
HSP90B1
Immunogen:
Purified glucose regulated protein 94 (grp94) from chicken oviducts
Quantity:
0.02 mg
Research Discipline:
Cancer, Cellular Markers, ER Markers, Golgi Apparatus Markers, Hypoxia, Membrane Trafficking and Chaperones
Gene ID (Entrez):
7184
Target Species:
Human, Mouse, Rat, Porcine, Canine, Chicken, Equine, Guinea Pig, Hamster, Monkey, Primate, Rabbit, Sheep, Xenopus species
Form:
Purified