NBP247949B
Vimentin Antibody (LN-6) - Azide and BSA Free, Novus Biologicals™
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
Vimentin
Classification
Monoclonal
Concentration
1.0 mg/mL
Dilution
Flow Cytometry : 0.5 - 1.0 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 1 - 2 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias
FLJ36605, vimentin
Host Species
Mouse
Purification Method
Protein A or G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype
IgM
Applications
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone
LN-6
Conjugate
Unconjugated
Formulation
PBS with No Preservative
Gene Symbols
VIM
Immunogen
Human thymic nuclear extract
Quantity
0.2 mg
Research Discipline
Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells
Gene ID (Entrez)
7431
Target Species
Human, Mouse, Rat, Porcine, Feline, Primate, Rabbit, Sheep
Form
Purified
Related Products
Description
- Vimentin Monoclonal specifically detects Vimentin in Human, Mouse, Rat, Porcine, Bovine, Feline, Rabbit, Sheep samples
- It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, CyTOF-ready.
Compare Similar Items
Show Difference
Antigen: Vimentin
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Flow Cytometry : 0.5 - 1.0 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 1 - 2 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias: FLJ36605, vimentin
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgM
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone: LN-6
Conjugate: Unconjugated
Formulation: PBS with No Preservative
Gene Symbols: VIM
Immunogen: Human thymic nuclear extract
Quantity: 0.2 mg
Research Discipline: Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells
Gene ID (Entrez): 7431
Target Species: Human, Mouse, Rat, Porcine, Feline, Primate, Rabbit, Sheep
Form: Purified
Antigen:
Vimentin
Classification:
Monoclonal
Concentration:
1.0 mg/mL
Dilution:
Flow Cytometry : 0.5 - 1.0 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 1 - 2 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias:
FLJ36605, vimentin
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgM
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone:
LN-6
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
VIM
Immunogen:
Human thymic nuclear extract
Quantity:
0.2 mg
Research Discipline:
Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells
Gene ID (Entrez):
7431
Target Species:
Human, Mouse, Rat, Porcine, Feline, Primate, Rabbit, Sheep
Form:
Purified
Antigen: Vimentin
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Western Blot : 0.25 - 0.5 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Gene Alias: FLJ36605, vimentin
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone: VM1170
Conjugate: Unconjugated
Formulation: PBS with No Preservative
Gene Symbols: VIM
Immunogen: Recombinant full-length human vimentin protein
Quantity: 0.1 mg
Research Discipline: Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells
Gene ID (Entrez): 7431
Target Species: Human, Mouse (Negative), Rat (Negative)
Form: Purified
Antigen:
Vimentin
Classification:
Monoclonal
Concentration:
1.0 mg/mL
Dilution:
Western Blot : 0.25 - 0.5 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Gene Alias:
FLJ36605, vimentin
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG1
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone:
VM1170
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
VIM
Immunogen:
Recombinant full-length human vimentin protein
Quantity:
0.1 mg
Research Discipline:
Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells
Gene ID (Entrez):
7431
Target Species:
Human, Mouse (Negative), Rat (Negative)
Form:
Purified
Antigen: Vimentin
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Western Blot : 0.25 - 0.5 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Gene Alias: FLJ36605, vimentin
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone: VM1170
Conjugate: Unconjugated
Formulation: PBS with No Preservative
Gene Symbols: VIM
Immunogen: Recombinant full-length human vimentin protein
Quantity: 0.2 mg
Research Discipline: Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells
Gene ID (Entrez): 7431
Target Species: Human, Mouse (Negative), Rat (Negative)
Form: Purified
Antigen:
Vimentin
Classification:
Monoclonal
Concentration:
1.0 mg/mL
Dilution:
Western Blot : 0.25 - 0.5 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Gene Alias:
FLJ36605, vimentin
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG1
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone:
VM1170
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
VIM
Immunogen:
Recombinant full-length human vimentin protein
Quantity:
0.2 mg
Research Discipline:
Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells
Gene ID (Entrez):
7431
Target Species:
Human, Mouse (Negative), Rat (Negative)
Form:
Purified
Antigen: Fc gamma RIIIA/CD16a
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias: CD16a, CD16a antigen, CD16FCRIIIA, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG, low affinity IIIa, receptor for (CD16), Fc gamma receptor III-A, FCG3, Fc-gamma receptor III-2 (CD 16), Fc-gamma receptor IIIb (CD16), Fc-gamma RIII, Fc-gamma RIIIa, Fc-gamma RIII-alpha, FCGR3, FCGRIII, FCR-10, FcRIII, FcRIIIa, IGFR3FcgammaRIIIA, immunoglobulin G Fc receptor III, low affinity III, receptor for (CD16), low affinity immunoglobulin gamma Fc region receptor III-A, neutrophil-specific antigen NA
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: It recognizes CD16 (Fcgamma RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (Fcgamma RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1 κ
Applications: Flow Cytometry, Immunofluorescence, CyTOF
Clone: ICO-116
Conjugate: Unconjugated
Formulation: PBS with No Preservative
Gene Symbols: FCGR3A
Immunogen: Human CD16 protein
Quantity: 0.1 mg
Research Discipline: Astrocyte Markers, Cellular Markers, Cytokine Research, Cytoskeleton Markers, Growth and Development, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers
Gene ID (Entrez): 2214
Target Species: Human
Form: Purified
Antigen:
Fc gamma RIIIA/CD16a
Classification:
Monoclonal
Concentration:
1.0 mg/mL
Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias:
CD16a, CD16a antigen, CD16FCRIIIA, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG, low affinity IIIa, receptor for (CD16), Fc gamma receptor III-A, FCG3, Fc-gamma receptor III-2 (CD 16), Fc-gamma receptor IIIb (CD16), Fc-gamma RIII, Fc-gamma RIIIa, Fc-gamma RIII-alpha, FCGR3, FCGRIII, FCR-10, FcRIII, FcRIIIa, IGFR3FcgammaRIIIA, immunoglobulin G Fc receptor III, low affinity III, receptor for (CD16), low affinity immunoglobulin gamma Fc region receptor III-A, neutrophil-specific antigen NA
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
It recognizes CD16 (Fcgamma RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (Fcgamma RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG1 κ
Applications:
Flow Cytometry, Immunofluorescence, CyTOF
Clone:
ICO-116
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
FCGR3A
Immunogen:
Human CD16 protein
Quantity:
0.1 mg
Research Discipline:
Astrocyte Markers, Cellular Markers, Cytokine Research, Cytoskeleton Markers, Growth and Development, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers
Gene ID (Entrez):
2214
Target Species:
Human
Form:
Purified