NBP247950B
Vimentin Antibody (VM1170) - Azide and BSA Free, Novus Biologicals™
Manufacturer: Fischer Scientific
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Antigen
Vimentin
Classification
Monoclonal
Concentration
1.0 mg/mL
Dilution
Western Blot : 0.25 - 0.5 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Gene Alias
FLJ36605, vimentin
Host Species
Mouse
Purification Method
Protein A or G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype
IgG1
Applications
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone
VM1170
Conjugate
Unconjugated
Formulation
PBS with No Preservative
Gene Symbols
VIM
Immunogen
Recombinant full-length human vimentin protein
Quantity
0.2 mg
Research Discipline
Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells
Gene ID (Entrez)
7431
Target Species
Human, Mouse (Negative), Rat (Negative)
Form
Purified
Related Products
Description
- Vimentin Monoclonal specifically detects Vimentin in Human, Mouse (Negative), Rat (Negative) samples
- It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.
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Antigen: Vimentin
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Western Blot : 0.25 - 0.5 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Gene Alias: FLJ36605, vimentin
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone: VM1170
Conjugate: Unconjugated
Formulation: PBS with No Preservative
Gene Symbols: VIM
Immunogen: Recombinant full-length human vimentin protein
Quantity: 0.2 mg
Research Discipline: Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells
Gene ID (Entrez): 7431
Target Species: Human, Mouse (Negative), Rat (Negative)
Form: Purified
Antigen:
Vimentin
Classification:
Monoclonal
Concentration:
1.0 mg/mL
Dilution:
Western Blot : 0.25 - 0.5 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Gene Alias:
FLJ36605, vimentin
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG1
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone:
VM1170
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
VIM
Immunogen:
Recombinant full-length human vimentin protein
Quantity:
0.2 mg
Research Discipline:
Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells
Gene ID (Entrez):
7431
Target Species:
Human, Mouse (Negative), Rat (Negative)
Form:
Purified
Antigen: Fc gamma RIIIA/CD16a
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias: CD16a, CD16a antigen, CD16FCRIIIA, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG, low affinity IIIa, receptor for (CD16), Fc gamma receptor III-A, FCG3, Fc-gamma receptor III-2 (CD 16), Fc-gamma receptor IIIb (CD16), Fc-gamma RIII, Fc-gamma RIIIa, Fc-gamma RIII-alpha, FCGR3, FCGRIII, FCR-10, FcRIII, FcRIIIa, IGFR3FcgammaRIIIA, immunoglobulin G Fc receptor III, low affinity III, receptor for (CD16), low affinity immunoglobulin gamma Fc region receptor III-A, neutrophil-specific antigen NA
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: It recognizes CD16 (Fcgamma RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (Fcgamma RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1 κ
Applications: Flow Cytometry, Immunofluorescence, CyTOF
Clone: ICO-116
Conjugate: Unconjugated
Formulation: PBS with No Preservative
Gene Symbols: FCGR3A
Immunogen: Human CD16 protein
Quantity: 0.1 mg
Research Discipline: Astrocyte Markers, Cellular Markers, Cytokine Research, Cytoskeleton Markers, Growth and Development, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers
Gene ID (Entrez): 2214
Target Species: Human
Form: Purified
Antigen:
Fc gamma RIIIA/CD16a
Classification:
Monoclonal
Concentration:
1.0 mg/mL
Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias:
CD16a, CD16a antigen, CD16FCRIIIA, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG, low affinity IIIa, receptor for (CD16), Fc gamma receptor III-A, FCG3, Fc-gamma receptor III-2 (CD 16), Fc-gamma receptor IIIb (CD16), Fc-gamma RIII, Fc-gamma RIIIa, Fc-gamma RIII-alpha, FCGR3, FCGRIII, FCR-10, FcRIII, FcRIIIa, IGFR3FcgammaRIIIA, immunoglobulin G Fc receptor III, low affinity III, receptor for (CD16), low affinity immunoglobulin gamma Fc region receptor III-A, neutrophil-specific antigen NA
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
It recognizes CD16 (Fcgamma RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (Fcgamma RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG1 κ
Applications:
Flow Cytometry, Immunofluorescence, CyTOF
Clone:
ICO-116
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
FCGR3A
Immunogen:
Human CD16 protein
Quantity:
0.1 mg
Research Discipline:
Astrocyte Markers, Cellular Markers, Cytokine Research, Cytoskeleton Markers, Growth and Development, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers
Gene ID (Entrez):
2214
Target Species:
Human
Form:
Purified
Antigen: Fc gamma RIIIA/CD16a
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias: CD16a, CD16a antigen, CD16FCRIIIA, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG, low affinity IIIa, receptor for (CD16), Fc gamma receptor III-A, FCG3, Fc-gamma receptor III-2 (CD 16), Fc-gamma receptor IIIb (CD16), Fc-gamma RIII, Fc-gamma RIIIa, Fc-gamma RIII-alpha, FCGR3, FCGRIII, FCR-10, FcRIII, FcRIIIa, IGFR3FcgammaRIIIA, immunoglobulin G Fc receptor III, low affinity III, receptor for (CD16), low affinity immunoglobulin gamma Fc region receptor III-A, neutrophil-specific antigen NA
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: It recognizes CD16 (Fcgamma RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (Fcgamma RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1 κ
Applications: Flow Cytometry, Immunofluorescence, CyTOF
Clone: ICO-116
Conjugate: Unconjugated
Formulation: PBS with No Preservative
Gene Symbols: FCGR3A
Immunogen: Human CD16 protein
Quantity: 0.2 mg
Research Discipline: Astrocyte Markers, Cellular Markers, Cytokine Research, Cytoskeleton Markers, Growth and Development, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers
Gene ID (Entrez): 2214
Target Species: Human
Form: Purified
Antigen:
Fc gamma RIIIA/CD16a
Classification:
Monoclonal
Concentration:
1.0 mg/mL
Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias:
CD16a, CD16a antigen, CD16FCRIIIA, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG, low affinity IIIa, receptor for (CD16), Fc gamma receptor III-A, FCG3, Fc-gamma receptor III-2 (CD 16), Fc-gamma receptor IIIb (CD16), Fc-gamma RIII, Fc-gamma RIIIa, Fc-gamma RIII-alpha, FCGR3, FCGRIII, FCR-10, FcRIII, FcRIIIa, IGFR3FcgammaRIIIA, immunoglobulin G Fc receptor III, low affinity III, receptor for (CD16), low affinity immunoglobulin gamma Fc region receptor III-A, neutrophil-specific antigen NA
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
It recognizes CD16 (Fcgamma RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (Fcgamma RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG1 κ
Applications:
Flow Cytometry, Immunofluorescence, CyTOF
Clone:
ICO-116
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
FCGR3A
Immunogen:
Human CD16 protein
Quantity:
0.2 mg
Research Discipline:
Astrocyte Markers, Cellular Markers, Cytokine Research, Cytoskeleton Markers, Growth and Development, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers
Gene ID (Entrez):
2214
Target Species:
Human
Form:
Purified
Antigen: CD45RB
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias: B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: CD45R, also designated CD45 and PTPRC, is identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1 κ
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone: PTPRC/1132
Conjugate: Unconjugated
Formulation: PBS with No Preservative
Gene Symbols: PTPRC
Immunogen: Recombinant full-length human CD45RB protein
Quantity: 0.1 mg
Research Discipline: Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Gene ID (Entrez): 5788
Target Species: Human, Monkey (Negative), Rat (Negative)
Form: Purified
Antigen:
CD45RB
Classification:
Monoclonal
Concentration:
1.0 mg/mL
Dilution:
Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias:
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
CD45R, also designated CD45 and PTPRC, is identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG1 κ
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone:
PTPRC/1132
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
PTPRC
Immunogen:
Recombinant full-length human CD45RB protein
Quantity:
0.1 mg
Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Gene ID (Entrez):
5788
Target Species:
Human, Monkey (Negative), Rat (Negative)
Form:
Purified