DEFA1 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: ACYCRIPACI AGERRYGTCI YQGRLWAFCC
• Preparation: Produced from sera of goats immunized with highly pure Recombinant Human NP-1
• Anti-Human NP-1-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect Human NP-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NP-1 (500-P126G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human NP-1
• Western Blot: To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hNP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P126GBT-1MG will be provided as 2 x 500 μg Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense
• They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina
• Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif
• The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense
• Several alpha defensin genes are clustered on chromosome 8
• This gene differs from defensin, alpha 3 by only one amino acid
• This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.