CD154 (CD40 Ligand) (soluble) Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
• Preparation: Produced from sera of goats immunized with highly pure Recombinant Human sCD40 Ligand
• Anti-Human sCD40 Ligand-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect Human sCD40 Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sCD40 Ligand (500-P142G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sCD40 Ligand
• Western Blot: To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsCD40L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P142GBT-1MG will be provided as 2 x 500 μg CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells
• The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines
• CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes
• It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.