FGF10 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS
• Preparation: Produced from sera of goats immunized with highly pure Recombinant Human FGF-10
• Anti-Human FGF-10-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect Human FGF-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human FGF-10 (500-P151G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-10
• Western Blot: To detect hFGF-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hFGF-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P151GBT-1MG will be provided as 2 x 500 μg FGF10 is a member of the fibroblast growth factor (FGF) family
• FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion
• This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7
• Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation
• This protein is also implicated to be a primary factor in the process of wound healing.