PDE6B Polyclonal Antibody, Invitrogen™

Description

• PA1-722 detects phosphodiesterase 6 beta (PDE6 beta) from mouse retinal extracts
• PA1-722 has been successfully used in Western blot procedures
• By Western blot, this antibody detects an ∽90 kDa protein representing PDE6 beta from mouse retinal extracts
• The PA1-722 immunizing peptides correspond to amino acid residues 20-36 from mouse and bovine PDE6 beta protein
• These peptides (Cat
• # PEP-183) are available for use in neutralization and control experiments
• The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways
• Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs)
• PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates
• PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system
• There are five different subunits consisting of rod and cone specific catalytic subunits: alpha' (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family)
• The catalytic core of the PDE6 system is comprised of alpha'/alpha' homodimers in the cone and alpha/beta heterodimers in the rod
• The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association.