CREB Polyclonal Antibody, Invitrogen™

Description

• PA1-850 detects both the phosphorylated and non-phosphorylated forms of cyclic-AMP response element binding protein (CREB) from rat, mouse, and human samples
• PA1-850 has been successfully used in ChIP, Western blot, ICC/IF and IHC-P procedures
• By Western blot, this antibody detects an ∽43 kDa protein representing CREB from GH4 cell extract
• The PA1-850 immunogen is a synthetic peptide corresponding to residues K(123) R R E I L S R R P S Y R K(136) of human CREB
• This sequence is completely conserved between human, bovine, canine, mouse, pig, sheep, and rat CREB protein
• The PA1-850 immunizing peptide (Cat
• # PEP-142) is available for use in neutralization and control experiments
• CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors
• Activation of CREB is dependent upon the phosphorylation of serine 133
• Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1
• Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor
• CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133
• CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory
• In humans, the gene is located on the q arm of chromosome 2
• Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.