SMAD1 Recombinant Rabbit Monoclonal Antibody (12H1L14), Invitrogen™

Description

• This antibody is predicted to react with equine, mouse, rabbit and rat based on sequence homology
• Intact IgG appears on a non-reducing gel as ∽150 kDa band and upon reduction generating a ∽25 kDa light chain band and a ∽50 kDa heavy chain
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
• The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
• Then, individual clones are screened to select the best candidates for production
• The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
• The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C
• elegans gene Sma
• SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways
• This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses
• In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase
• The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation
• This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation
• Alternatively spliced transcript variants encoding the same protein have been observed.