TAP Tag Polyclonal Antibody, Invitrogen™

Description

• CAB1001 detects TAP
• CAB1001 has been successfully used in Western Blot, IP, and ChIP applications The CAB1001 immunogen is a KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage
• TAP adds about 20 kDa to the size of the protein
• Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available
• Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins
• The protocol involves the fusion of the &quoteTAP tag&quote (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest
• The TAP technique is useful in analyzing in vivo interactions.