CD4 Monoclonal Antibody (RPA-T4), Invitrogen™

Description

• The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing
• We recommend the use of acetone fixation for frozen sections
• A suggested positive control for immunohistochemical applications is human lymphatic tissue
• For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL
• The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV
• CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells
• At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues
• CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck
• It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system
• Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].