BACE1 Recombinant Rabbit Monoclonal Antibody (027), Invitrogen™

Description

• This product is preservative free
• It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%)
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
• The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
• Then, individual clones are screened to select the best candidates for production
• The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
• This antibody has specificity for Human BACE1
• BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimer's disease (AD)
• In some cases early onset familial AD can be attributed to a "Swedish" mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1
• This and other genetic and pathological evidence has led to therapeutic approaches that have focused on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase stuff
• APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99)
• The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all sufferers of Alzheimer's disease
• The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2)
• bACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.