CD146 Recombinant Rabbit Monoclonal Antibody (5), Invitrogen™

Description

• This product is preservative free
• It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%)
• Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
• The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
• Then, individual clones are screened to select the best candidates for production
• The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
• This antibody has specificity for Human CD146/MCAM
• CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily
• CD146 is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types
• In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly
• CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue
• CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread
• Further, CD146 could be an adhesion molecule active in neural crest cells during embryonic development
• CD146 acts also as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.