PIMA533032

6x-His Tag Recombinant Rabbit Monoclonal Antibody (RM146), Invitrogen™

Manufacturer: Thermo Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 PIMA533032-Each-of-1 In Stock ₹ 45,731.82

PIMA533032 - Each of 1

₹ 45,731.82

In Stock

Quantity

1

Base Price: ₹ 45,731.82

GST (18%): ₹ 8,231.728

Total Price: ₹ 53,963.548

Antigen

6x-His Tag

Classification

Recombinant Monoclonal

Concentration

1.0 mg/mL

Formulation

PBS with 1% BSA, 50% glycerol and 0.09% sodium azide

Host Species

Rabbit

Purification Method

Protein A

Regulatory Status

RUO

Target Species

Tag

Product Type

Antibody

Isotype

IgG

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Clone

RM146

Conjugate

Unconjugated

Gene Alias

6His tag, 6X His, His Tag, Histidine Tag

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Quantity

100 μg

Primary or Secondary

Primary

Content And Storage

-20°C

Form

Liquid

Target Molecule

Tag

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Description

  • Specificity: reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus
  • No cross reactivity with other endogenous protein in mammalian or bacteria cells
  • Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
  • The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
  • Then, individual clones are screened to select the best candidates for production
  • The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
  • The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH)
  • The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography
  • Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering
  • By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available
  • His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins
  • For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent
  • His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

Compare Similar Items

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Thermo Scientific

PIMA533032

--


Antigen:
6x-His Tag

Classification:
Recombinant Monoclonal

Concentration:
1.0 mg/mL

Formulation:
PBS with 1% BSA, 50% glycerol and 0.09% sodium azide

Host Species:
Rabbit

Purification Method:
Protein A

Regulatory Status:
RUO

Target Species:
Tag

Product Type:
Antibody

Isotype:
IgG

Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Clone:
RM146

Conjugate:
Unconjugated

Gene Alias:
6His tag, 6X His, His Tag, Histidine Tag

Immunogen:
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Quantity:
100 μg

Primary or Secondary:
Primary

Content And Storage:
-20°C

Form:
Liquid

Target Molecule:
Tag

Img

Thermo Scientific

PIMA533033

--


Antigen:
delta Catenin

Classification:
Recombinant Monoclonal

Concentration:
0.5 mg/mL

Formulation:
PBS with 1% BSA, 50% glycerol and 0.09% sodium azide

Host Species:
Rabbit

Purification Method:
Protein A

Regulatory Status:
RUO

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG

Applications:
Immunohistochemistry (Paraffin), Western Blot

Clone:
RM353

Conjugate:
Unconjugated

Gene Alias:
AA409437, AU019353, cadherin-associated Src substrate, Cadherin-associated Src substrate (CAS), CAS, Catenin (cadherin associated protein) delta 1, catenin (cadherin associated protein), delta 1, catenin (cadherin-associated protein), delta 1, catenin delta 1, catenin delta-1, catenin src, catenin, delta 1, Catns, CTNND, Ctnnd1, hypothetical protein, Kiaa0384, mKIAA0384, p120, p120 catenin, P120 CTN, P120 p120, P120 P120CTN, p120(CAS), p120(ctn), P120CAS, p120-catenin, P120CTN

Immunogen:
A peptide corresponding to the C-terminus of human p120 catenin (Catenin delta-1).

Quantity:
100 μL

Primary or Secondary:
Primary

Content And Storage:
-20°C

Form:
Liquid

Target Molecule:
__

Img

Thermo Scientific

PIMA533034

--


Antigen:
NAPSA

Classification:
Recombinant Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 1% BSA, 50% glycerol and 0.09% sodium azide

Host Species:
Rabbit

Purification Method:
Protein A

Regulatory Status:
RUO

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG

Applications:
Immunohistochemistry (Paraffin), Western Blot

Clone:
RM366

Conjugate:
Unconjugated

Gene Alias:
asp 4, ASP4, Aspartyl protease 4, KAP, Kdap, KDAP-1, kidney-derived aspartic protease-like protein, Nap, NAP1, NAPA, Napsa, napsin A aspartic peptidase, napsin-1, Napsin-A, pronapsin, pronapsin A, SNAPA, TA01/TA02

Immunogen:
A peptide corresponding to the N-terminus of human Napsin-A.

Quantity:
100 μL

Primary or Secondary:
Primary

Content And Storage:
-20°C

Form:
Liquid

Target Molecule:
__

Img

Thermo Scientific

PIMA533035

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Antigen:
c-Fos

Classification:
Recombinant Monoclonal

Concentration:
0.05 mg/mL

Formulation:
PBS with 1% BSA, 50% glycerol and 0.09% sodium azide

Host Species:
Rabbit

Purification Method:
Protein A

Regulatory Status:
RUO

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG

Applications:
Immunohistochemistry (Paraffin), Western Blot

Clone:
RM374

Conjugate:
Unconjugated

Gene Alias:
activator protein 1, AP-1, cellular oncogene c-fos, cellular oncogene fos, cFos, C-FOS, c-fos oncogene, c-fos protooncogene protein, D12Rfj1, FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, FBJ murine osteosarcoma viral oncogene homolog B, FBJ osteosarcoma oncogene, FBJ osteosarcoma oncogene B, FBJ osteosarcoma viral oncogene isoform deltaFosb-2, FOS, Fos proto-oncogene, AP-1 trancription factor subunit, Fos proto-oncogene, AP-1 transcription factor subunit, Fosb, FosB proto-oncogene, AP-1 transcription factor subunit, fra-2, G0/G1 switch regulatory protein 7, G0S7, I79_009428, p55, protein fosB, proto-oncogene cFOS, proto-oncogene c-Fos, proto-oncogene protein c-fos, v-fos FBJ murine osteosarcoma viral oncogene homolog

Immunogen:
A peptide corresponding to the N-terminus of human Proto-oncogene c-Fos.

Quantity:
100 μL

Primary or Secondary:
Primary

Content And Storage:
-20°C

Form:
Liquid

Target Molecule:
__