PIMA547746

6x His Tag Chimeric Recombinant Mouse Monoclonal Antibody (3D5), Invitrogen™

Manufacturer: Thermo Scientific

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Pack Size SKU Availability Price
Each of 1 PIMA547746-Each-of-1 In Stock ₹ 44,961.78

PIMA547746 - Each of 1

₹ 44,961.78

In Stock

Quantity

1

Base Price: ₹ 44,961.78

GST (18%): ₹ 8,093.12

Total Price: ₹ 53,054.90

Antigen

6x His Tag Chimeric

Classification

Recombinant Monoclonal

Concentration

1 mg/mL

Formulation

PBS with 0.02% ProClin 300

Gene Accession No.

P01106

Host Species

Mouse

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

4609

Content And Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Isotype

IgG1 κ

Applications

ELISA, Western Blot

Clone

3D5

Conjugate

Unconjugated

Gene

MYC

Gene Alias

6His tag; 6X His; His Tag; Histidine Tag

Immunogen

Purified his tagged Fab fragment.

Quantity

200 μg

Primary or Secondary

Primary

Target Species

Tag

Form

Liquid

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Description

  • Specificity: This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins
  • It also has affinity for internal His-tags but no affinity for N-terminal His-tags
  • The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH)
  • The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography
  • Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering
  • By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available
  • His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins
  • For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent
  • His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

Compare Similar Items

Show Difference

Img

Thermo Scientific

PIMA547746

--


Antigen:
6x His Tag Chimeric

Classification:
Recombinant Monoclonal

Concentration:
1 mg/mL

Formulation:
PBS with 0.02% ProClin 300

Gene Accession No.:
P01106

Host Species:
Mouse

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
4609

Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Isotype:
IgG1 κ

Applications:
ELISA, Western Blot

Clone:
3D5

Conjugate:
Unconjugated

Gene:
MYC

Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag

Immunogen:
Purified his tagged Fab fragment.

Quantity:
200 μg

Primary or Secondary:
Primary

Target Species:
Tag

Form:
Liquid

Img

Thermo Scientific

PIMA547748

--


Antigen:
6x His Tag Chimeric

Classification:
Recombinant Monoclonal

Concentration:
1 mg/mL

Formulation:
PBS with 0.02% ProClin 300

Gene Accession No.:
__

Host Species:
Rabbit

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
__

Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Isotype:
IgG κ

Applications:
ELISA, Immunocytochemistry, Western Blot

Clone:
3D5

Conjugate:
Unconjugated

Gene:
__

Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag

Immunogen:
Purified his tagged Fab fragment.

Quantity:
200 μg

Primary or Secondary:
Primary

Target Species:
Tag

Form:
Liquid

Img

Thermo Scientific

PIMA547749

--


Antigen:
6x His Tag Chimeric

Classification:
Recombinant Monoclonal

Concentration:
1 mg/mL

Formulation:
PBS with 0.02% ProClin 300

Gene Accession No.:
__

Host Species:
Rat

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
__

Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Isotype:
IgG1 κ

Applications:
ELISA, Western Blot

Clone:
3D5

Conjugate:
Unconjugated

Gene:
__

Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag

Immunogen:
Purified his tagged Fab fragment.

Quantity:
200 μg

Primary or Secondary:
Primary

Target Species:
Tag

Form:
Liquid

Img

Thermo Scientific

PIMA547750

--


Antigen:
FITC Chimeric

Classification:
Recombinant Monoclonal

Concentration:
1 mg/mL

Formulation:
PBS with 0.02% ProClin 300

Gene Accession No.:
__

Host Species:
Mouse

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
__

Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Isotype:
IgG1 κ

Applications:
Functional Assay

Clone:
4-4-20 (enhanced)

Conjugate:
Unconjugated

Gene:
__

Gene Alias:
Fluorescein

Immunogen:
Fluorescein I-bovine serum albumin (FlI-BSA).

Quantity:
200 μg

Primary or Secondary:
Primary

Target Species:
Chemical

Form:
Liquid