CD200R3 Monoclonal Antibody (Ba103), Invitrogen™

Description

• Product is supplied at 1 mL (100 μL/mL)
• This antibody recognizes mouse CD200 Receptor-like 3 (CD200R3), a protein of ∽38 kDa or ∽74 kDa (reduced or non-reduced condition, respectively)
• Application Notes: Western Blot - Antibody was used in reducing and non-reducing conditions with MC/9 lysates
• Under reducing conditions, the resulting band size was 38kDa, and under non-reducing conditions, the resulting band size was 74 kDa
• Flow Cytometry - Basophils and mast cells were used
• No fixation is needed
• Functional Studies - Antibody was used as an agonist in basophil-mediated allergic reactions
• A single intravenous administration of 30 μg antibody was used
• The biological activity of the antibodyis a drastic reduction in the basophile number in the peripheral blood and spleen, to approximately 20% of the normal level
• This product is also useful for mast cell degranulation
• Readout was determined by measuring hexosaminidase activity
• Immunoprecipitation - mast cells were lysed with 1% NP-40 containing lysis buffer with proteinase inhibitor mixture before incubation with the antibody
• CD200R3 is a dimeric cell surface receptor also known as CD200RLb
• CD200R3 contains two Ig-like extracellular domains and as such is a member of the immunoglobulin superfamily of receptors
• The intracellular region of CD200R3 associates with the adaptor molecule DAP12, and possibly also DAP10, CD3 zeta and FcR gamma-chain, indicating that it is capable of transducing stimulatory signals
• In this respect CD200R3 bears similarity to activatory receptors CD200R2 and CD200R4
• In contrast, an inhibitory receptor CD200R (also known as CD200R1) relies on other adaptors
• CD200R3 is primarily expressed by basophils and mast cells, cells known to be involved in anti-parasitic immune responses and frequently also allergic reactions
• The surface expression of CD200R3 decreases when basophils are activated by ligating their CD16 and CD32
• Remarkably, most of this downregulation is observed within the first 30 minutes after activation
• Unlike CD200R, CD200R3 does not bind CD200.