SHANK1/SHANK3 Monoclonal Antibody (S367-51), FITC, Invitrogen™

Description

• Mouse: 100% identity (89/89 amino acids identical)
• Human: 97% identity (87/89 amino acids identical)
• ∽70% identity with SHANK1 and SHANK2
• 1 μg/mL of MA5-45674 was sufficient for detection of SHANK1/SHANK3 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.∣Detects approximately 190kDa
• Cross-reacts with SHANK1
• Does not cross-react with SHANK2
• Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance
• Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton
• Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters
• By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling
• Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity
• May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation
• {PubMed:21606927, PubMed:21795692, PubMed:23100419, PubMed:23897824, PubMed:24089484}.