CD146 Recombinant Rabbit Monoclonal Antibody (044), PE, Invitrogen™

Description

• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD146 (Met1-Gly559) and conjugated with PE under optimum conditions, the unreacted PE was removed
• Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity
• Protected from prolonged exposure to light
• Do not freeze! Sodium azide is toxic to cells and should be disposed of properly
• Flush with large volumes of water during disposal
• CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily
• CD146 is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types
• In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly
• CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue
• CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread
• Further, CD146 could be an adhesion molecule active in neural crest cells during embryonic development
• CD146 acts also as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.