CCRL2 Chimeric Recombinant Rabbit Monoclonal Antibody (BZ5B8), Invitrogen™

Description

• Specificity: This antibody is specific for murine CCRL2 (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells
• This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites
• CCRL2 presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23
• Chemerin can block anti-CCRL2 antibody binding
• Preparation: This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate
• The immunizing amino-terminal mCCRL2 peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1-24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH)
• CCRL2 (C-C chemokine receptor-like 2), also known as chemokine receptor X, human chemokine receptor (HCR), CRAM-A or CRAM-B, is a seven pass transmembrane protein expressed by monocytes, neutrophils, and dendritic cells of immunal tissues (predominanly spleen, fetal liver, bone marrow and lymph node)
• It shares over 40% homology with other C-C chemokine receptors but does not share the conserved DRY motif which is among the important motifs necessary for signalling and ligand-binding
• C-C chemokine receptors are G-protein coupled, seven pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to areas of inflammation.