alpha Internexin Polyclonal Antibody, Invitrogen™

Description

• In Western blot, a band at ∽66 kDa is detected
• Minor bands of approximately 50 kDa and 150 kDa are also detected, which most likely correspond to alpha-internexin breakdown products and covalent dimers, respectively
• alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development
• It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization
• Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H
• In some mature neurons a-internexin is the only neurofilament subunit expressed
• Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture
• In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage
• The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.