S-peptide Epitope Tag Polyclonal Antibody, Invitrogen™

Description

• Store as concentrated solution
• Centrifuge briefly prior to opening vial
• For short-term storage (1-2 weeks), store at 4°C
• For long-term storage, aliquot and store at -20°C or below
• Avoid multiple freeze-thaw cycles
• The S-Peptide epitope tag (S-Tag (TM)) can be used for numerous applications which include the analysis of localization, expression detection, and purification of polypeptides from mammalian cells
• The small size of the tag (15 aa), the ability to purify tagged polypeptides without antibodies, and the very low expression of endogenous tissues in mammalian cells that react with S-Peptide make the S-Peptide antibody an attractive alternative to many existing epitope tags
• Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes
• To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag
• The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag
• Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene.