MCL-1 Polyclonal Antibody, Invitrogen™

Description

• Positive test controls include: Mouse spleen, Rat lung, Rat spleen
• The target is usually found in the following locations: Cytoplasm, Membrane, Mitochondrion, Nucleus, Single-pass membrane protein, nucleoplasm
• Immunogen sequence: GGEAGAVIGG SAGASPPSTL TPDSRRVARP PPIGAEVPDV TATPARLLFF APTRRAAPLE EMEAPAADAI MSPEEELDGY EPEPLGKRPA VLPLLELVGE S MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programing, differentiation and concomitant maintenance of cell viability, but not of proliferation
• Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it
• The carboxy terminal of MCL1 and bcl-2 share significant sequence homology
• Expression of MCL1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g
• ionizing radiation, μgtraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2
• Enhanced expression of MCL1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation
• Like bcl-2, MCL1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis
• While the mechanism by which MCL1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak
• MCL1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types
• MCL1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals
• MCL1 exists as at least two distinct isoforms designated MCL1L and MCL1S
• In marked contrast to the larger isoform of MCL1, overexpression of MCL1S promotes cell death.