PIPA5143756
Phospho-A-Raf/B-Raf/C-Raf (Ser446, Ser338, Ser299) Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5143756-Each-of-1 | In Stock | ₹ 44,106.18 |
PIPA5143756 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,106.18
GST (18%): ₹ 7,939.112
Total Price: ₹ 52,045.292
Antigen
Phospho-A-Raf/B-Raf/C-Raf (Ser446, Ser338, Ser299)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
BRAF
Gene Alias
BRAF1; CRaf; Raf1; RAFB1
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
109880, 110157, 114486, 24703, 5894, 673
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Isotype
IgG
Applications
Western Blot
Concentration
Lot-Specific
Formulation
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.
P04049, P11345, P15056, P28028, Q99N57
Gene Symbols
BRAF, RAF1
Immunogen
Phospho-B-Raf (Ser-446) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding serine 446 in human B-Raf. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299).
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Form
Liquid
Related Products
Description
- This antibody was cross-adsorbed to phospho-B-Raf (Ser-579) peptide before affinity purification using phospho-B-Raf (Ser-446) peptide
- The purified antibody detects a strong band at 95 kDa* and a weaker band at 105 kDa in western blots of human Jurkat or A431 cells treated with Calyculin A
- The antibody also detects other phosphorylated proteins at 150 and 200 kDa in calyculin A treated cells
- The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation
- The Raf kinase family includes A-Raf, B-Raf, and C-Raf
- Each family member has three highly conserved regions (CR1-3)
- The N-terminal CR1 contains the Ras-GTP-binding domain
- The CR2 contains a negative regulatory serine residue (C-Raf (Ser259)/B-Raf(Ser365)) that may bind 14-3-3 proteins
- The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment
- Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites
- In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain
- Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation
- Ser-579 is required for growth factor activation and kinase activity
- Thus, multiple sites of phosphorylation within Rafs may be important for regulation of their activity.
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Antigen: Phospho-A-Raf/B-Raf/C-Raf (Ser446, Ser338, Ser299)
Classification: Polyclonal
Conjugate: Unconjugated
Gene: BRAF
Gene Alias: BRAF1; CRaf; Raf1; RAFB1
Host Species: Rabbit
Purification Method: Antigen affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 109880, 110157, 114486, 24703, 5894, 673
Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
Isotype: IgG
Applications: Western Blot
Concentration: Lot-Specific
Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.: P04049, P11345, P15056, P28028, Q99N57
Gene Symbols: BRAF, RAF1
Immunogen: Phospho-B-Raf (Ser-446) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding serine 446 in human B-Raf. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299).
Quantity: 100 μL
Primary or Secondary: Primary
Target Species: Human, Mouse, Rat
Form: Liquid
Antigen:
Phospho-A-Raf/B-Raf/C-Raf (Ser446, Ser338, Ser299)
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
BRAF
Gene Alias:
BRAF1; CRaf; Raf1; RAFB1
Host Species:
Rabbit
Purification Method:
Antigen affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
109880, 110157, 114486, 24703, 5894, 673
Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles
Isotype:
IgG
Applications:
Western Blot
Concentration:
Lot-Specific
Formulation:
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.:
P04049, P11345, P15056, P28028, Q99N57
Gene Symbols:
BRAF, RAF1
Immunogen:
Phospho-B-Raf (Ser-446) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding serine 446 in human B-Raf. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299).
Quantity:
100 μL
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Rat
Form:
Liquid
Antigen: Phospho-A-Raf/B-Raf/C-Raf (Ser471, Ser579, Ser432)
Classification: Polyclonal
Conjugate: Unconjugated
Gene: BRAF
Gene Alias: BRAF1; CRaf; Raf1; RAFB1
Host Species: Rabbit
Purification Method: Antigen affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 109880, 110157, 114486, 24703, 5894, 673
Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
Isotype: IgG
Applications: Western Blot
Concentration: Lot-Specific
Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.: P04049, P11345, P15056, P28028, Q99N57
Gene Symbols: BRAF, RAF1
Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-471 in human C-Raf. This sequence has high homology with similar regions in rat and mouse C-Raf, and has high homology to the conserved sites in B-Raf (Ser-579) and A-Raf (Ser-432).
Quantity: 100 μL
Primary or Secondary: Primary
Target Species: Human, Mouse, Rat
Form: Liquid
Antigen:
Phospho-A-Raf/B-Raf/C-Raf (Ser471, Ser579, Ser432)
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
BRAF
Gene Alias:
BRAF1; CRaf; Raf1; RAFB1
Host Species:
Rabbit
Purification Method:
Antigen affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
109880, 110157, 114486, 24703, 5894, 673
Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles
Isotype:
IgG
Applications:
Western Blot
Concentration:
Lot-Specific
Formulation:
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.:
P04049, P11345, P15056, P28028, Q99N57
Gene Symbols:
BRAF, RAF1
Immunogen:
A synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-471 in human C-Raf. This sequence has high homology with similar regions in rat and mouse C-Raf, and has high homology to the conserved sites in B-Raf (Ser-579) and A-Raf (Ser-432).
Quantity:
100 μL
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Rat
Form:
Liquid
Antigen: Phospho-RHOA (Ser188)
Classification: Polyclonal
Conjugate: Unconjugated
Gene: RHOA
Gene Alias: Aplysia ras-related homolog 12; Aplysia RAS-related homolog 6; aplysia ras-related homolog A; aplysia ras-related homolog A1; aplysia ras-related homolog A2; ARH12; ARH6; ARH9; ARHA; Arha1; Arha2; ARHB; ARHC; h12; h6; H9; MGC72339; MST081; MSTP081; oncogene RHO H12; oncogene RHO H6; oncogene RHO H9; plysia ras-related homolog A2; Ras family member A; ras homolog A1; ras homolog A2; ras homolog family member A; ras homolog family member B; ras homolog family member C; ras homolog gene family, member A; ras homolog gene family, member A1; ras homolog gene family, member A2; ras homolog gene family, member B; ras homolog gene family, member C; RAS-related homolog 9; Rho; Rho cDNA clone 12; Rho cDNA clone 6; rho cDNA clone 9; Rho family GTPase; RHO12; Rhoa; RHOB; RHOC; rhoC GTPase; RHOH12; RHOH6; RHOH9; rho-related GTP-binding protein RhoB; Rho-related GTP-binding protein RhoC; small GTP binding protein RhoA; small GTP binding protein RhoC; transforming protein RhoA
Host Species: Rabbit
Purification Method: Antigen affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 117273, 11848, 387
Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
Isotype: IgG
Applications: Western Blot
Concentration: 0.5 mg/mL
Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.: P61586, P61589, Q9QUI0
Gene Symbols: RHOA
Immunogen: Phospho-RhoA (Ser-188) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 188 of human RhoA. This peptide sequence is highly conserved in rat, mouse, and chicken RhoA, but is not found in RhoB or RhoC proteins.
Quantity: 100 μL
Primary or Secondary: Primary
Target Species: Human, Mouse, Rat
Form: Liquid
Antigen:
Phospho-RHOA (Ser188)
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
RHOA
Gene Alias:
Aplysia ras-related homolog 12; Aplysia RAS-related homolog 6; aplysia ras-related homolog A; aplysia ras-related homolog A1; aplysia ras-related homolog A2; ARH12; ARH6; ARH9; ARHA; Arha1; Arha2; ARHB; ARHC; h12; h6; H9; MGC72339; MST081; MSTP081; oncogene RHO H12; oncogene RHO H6; oncogene RHO H9; plysia ras-related homolog A2; Ras family member A; ras homolog A1; ras homolog A2; ras homolog family member A; ras homolog family member B; ras homolog family member C; ras homolog gene family, member A; ras homolog gene family, member A1; ras homolog gene family, member A2; ras homolog gene family, member B; ras homolog gene family, member C; RAS-related homolog 9; Rho; Rho cDNA clone 12; Rho cDNA clone 6; rho cDNA clone 9; Rho family GTPase; RHO12; Rhoa; RHOB; RHOC; rhoC GTPase; RHOH12; RHOH6; RHOH9; rho-related GTP-binding protein RhoB; Rho-related GTP-binding protein RhoC; small GTP binding protein RhoA; small GTP binding protein RhoC; transforming protein RhoA
Host Species:
Rabbit
Purification Method:
Antigen affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
117273, 11848, 387
Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles
Isotype:
IgG
Applications:
Western Blot
Concentration:
0.5 mg/mL
Formulation:
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.:
P61586, P61589, Q9QUI0
Gene Symbols:
RHOA
Immunogen:
Phospho-RhoA (Ser-188) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 188 of human RhoA. This peptide sequence is highly conserved in rat, mouse, and chicken RhoA, but is not found in RhoB or RhoC proteins.
Quantity:
100 μL
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Rat
Form:
Liquid
Antigen: C9orf126
Classification: Polyclonal
Conjugate: Unconjugated
Gene: SCAI
Gene Alias: 9330112M16; A930041I02Rik; AI662729; C9orf126; NET40; protein SCAI; SCAI; suppressor of cancer cell invasion; suppressor of cancer cell invasion protein
Host Species: Rabbit
Purification Method: Antigen affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 286205, 320271, 690538
Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
Isotype: IgG
Applications: Western Blot
Concentration: Lot-Specific
Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.: Q8C8N2, Q8N9R8
Gene Symbols: SCAI
Immunogen: SCAI synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of mouse SCAI. This peptide sequence is highly conserved in human, rat, mouse, and chicken SCAI.
Quantity: 100 μL
Primary or Secondary: Primary
Target Species: Human, Mouse, Rat
Form: Liquid
Antigen:
C9orf126
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
SCAI
Gene Alias:
9330112M16; A930041I02Rik; AI662729; C9orf126; NET40; protein SCAI; SCAI; suppressor of cancer cell invasion; suppressor of cancer cell invasion protein
Host Species:
Rabbit
Purification Method:
Antigen affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
286205, 320271, 690538
Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles
Isotype:
IgG
Applications:
Western Blot
Concentration:
Lot-Specific
Formulation:
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.:
Q8C8N2, Q8N9R8
Gene Symbols:
SCAI
Immunogen:
SCAI synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of mouse SCAI. This peptide sequence is highly conserved in human, rat, mouse, and chicken SCAI.
Quantity:
100 μL
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Rat
Form:
Liquid