E-cadherin Polyclonal Antibody, Invitrogen™

Description

• Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min
• Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature
• A suggested positive control is skin, tonsil or breast tissue
• E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily
• The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail
• Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer
• Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis
• The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization
• Identified transcript variants arise from mutation at consensus splice sites
• E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity
• In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16
• Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.