HIF-1 beta Polyclonal Antibody, Invitrogen™

Description

• Keep as concentrated solution
• Predicted reactivity: Mouse (84%), Rat (82%), Pig (91%), Rabbit (86%), Sheep (91%), Bovine (92%), Guinea pig (92%)
• Positive Control: 293T, A431, HeLa, HepG2 (untreated), HepG2 ( 1% O2 treatment for 24hr)
• Store product as a concentrated solution
• Centrifuge briefly prior to opening the vial
• HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products
• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease
• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease
• HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis
• This occurs as a posttranslational modification by prolyl hydroxylation
• HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits
• Both subunits are constantly translated
• However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes
• Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity
• Therefore, the concentration of HIF-1 alpha increases in the cell
• In contrast, HIF-1 beta remains stable under either condition
• HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products
• Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products
• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease
• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease
• HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis
• This occurs as a posttranslational modification by prolyl hydroxylation
• HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits
• Both subunits are constantly translated
• However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes
• Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity
• Therefore, the concentration of HIF-1 alpha increases in the cell
• In contrast, HIF-1 beta remains stable under either condition
• HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products
• Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.