PIPA588580
RAG2 Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA588580-Each-of-1 | In Stock | ₹ 44,705.10 |
PIPA588580 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,705.10
GST (18%): ₹ 8,046.918
Total Price: ₹ 52,752.018
Antigen
RAG2
Classification
Polyclonal
Conjugate
Unconjugated
Gene
RAG2
Gene Alias
RAG 2, Rag2, RAG-2, recombinase activating gene 2, recombination activating gene 2, recombination activating protein 2, V(D)J recombination-activating protein 2
Host Species
Rabbit
Purification Method
Affinity Chromatography
Regulatory Status
RUO
Gene ID (Entrez)
19374, 5897
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Applications
Western Blot
Concentration
0.7 mg/mL
Formulation
PBS with 50% glycerol and 0.01% thimerosal, pH 7.3
Gene Accession No.
P21784, P55895
Gene Symbols
RAG2
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 258-527 of human RAG2 (NP_0012307151)
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG
Related Products
Description
- Positive Samples: Mouse testis, Mouse liver; Cellular Location: Nucleus Immunogen sequence: VSSAILTQTN NDEFVIVGGY QLENQKRMIC NIISLEDNKI EIREMETPDW TPDIKHSKIW FGSNMGNGTV FLGIPGDNKQ VVSEGFYFYM LKCAEDDTNE EQTTFTNSQT STEDPGDSTP FEDSEEFCFS AEANSFDGDD EFDTYNEDDE EDESETGYWI TCCPTCDVDI NTWVPFYSTE LNKPAMIYCS HGDGHWVHAQ CMDLAERTLI HLSAGSNKYY CNEHVEIARA LHTPQRVLPL KKPPMKSLRK KGSGKILTPA KKSFLRRLFD RAG2 (V(D)J recombination-activating protein 2) is a core component of the RAG complex - a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination
- V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity) and J (joining) gene segments
- DNA cleavage by the RAG complex occurs in 2 steps: 1) first a nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends
- 2) hairpin coding ends and 2 blunt, 5'-phosphorylated ends are created
- The chromatin structure plays an essential rold in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and hair pinning expressed on individual B-lymphocytes
- The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin
- This prevents accessibility to the RAG complex and recombination of the second allele
- In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1
- Mutations affecting the gene can results in combined cellular and humoral immune defects with granulomas, severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive, and Omenn syndrome.
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Antigen: RAG2
Classification: Polyclonal
Conjugate: Unconjugated
Gene: RAG2
Gene Alias: RAG 2, Rag2, RAG-2, recombinase activating gene 2, recombination activating gene 2, recombination activating protein 2, V(D)J recombination-activating protein 2
Host Species: Rabbit
Purification Method: Affinity Chromatography
Regulatory Status: RUO
Gene ID (Entrez): 19374, 5897
Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
Form: Liquid
Applications: Western Blot
Concentration: 0.7 mg/mL
Formulation: PBS with 50% glycerol and 0.01% thimerosal, pH 7.3
Gene Accession No.: P21784, P55895
Gene Symbols: RAG2
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 258-527 of human RAG2 (NP_0012307151)
Quantity: 100 μL
Primary or Secondary: Primary
Target Species: Human, Mouse
Product Type: Antibody
Isotype: IgG
Antigen:
RAG2
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
RAG2
Gene Alias:
RAG 2, Rag2, RAG-2, recombinase activating gene 2, recombination activating gene 2, recombination activating protein 2, V(D)J recombination-activating protein 2
Host Species:
Rabbit
Purification Method:
Affinity Chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
19374, 5897
Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Applications:
Western Blot
Concentration:
0.7 mg/mL
Formulation:
PBS with 50% glycerol and 0.01% thimerosal, pH 7.3
Gene Accession No.:
P21784, P55895
Gene Symbols:
RAG2
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 258-527 of human RAG2 (NP_0012307151)
Quantity:
100 μL
Primary or Secondary:
Primary
Target Species:
Human, Mouse
Product Type:
Antibody
Isotype:
IgG
Antigen: SHMT1
Classification: Polyclonal
Conjugate: Unconjugated
Gene: Shmt1
Gene Alias: AI324848, AI385541, C81125, CSHMT, cytoplasmic serine hydroxymethyltransferase, cytosolic form, cytosolic serine hydroxymethyltransferase, glycine hydroxymethyltransferase, LRRGT00032, MEL-32, MGC15229, MGC24556, mshmt, mshmt1, mshmt2, serine hydroxymethyl transferase, serine hydroxymethyltransferase, serine hydroxymethyltransferase 1, serine hydroxymethyltransferase 1 (soluble), serine hydroxymethyltransferase 1 (soluble) isoform 1, serine hydroxymethyltransferase 2 (mitochondrial), serine hydroxymethyltransferase, cytosolic, Serine methylase, SHMT, Shmt1, shmt1 protein, SHMT2, zgc:66171, zgc:77524, zSHMT-1
Host Species: Rabbit
Purification Method: Affinity Chromatography
Regulatory Status: RUO
Gene ID (Entrez): 20425, 287379, 6470
Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
Form: Liquid
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Concentration: 2.84 mg/mL
Formulation: PBS with 50% glycerol and 0.01% thimerosal, pH 7.3
Gene Accession No.: P34896, P50431
Gene Symbols: Shmt1
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 285-444 of human SHMT1 (NP_6837181)
Quantity: 100 μL
Primary or Secondary: Primary
Target Species: Human, Mouse, Rat
Product Type: Antibody
Isotype: IgG
Antigen:
SHMT1
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
Shmt1
Gene Alias:
AI324848, AI385541, C81125, CSHMT, cytoplasmic serine hydroxymethyltransferase, cytosolic form, cytosolic serine hydroxymethyltransferase, glycine hydroxymethyltransferase, LRRGT00032, MEL-32, MGC15229, MGC24556, mshmt, mshmt1, mshmt2, serine hydroxymethyl transferase, serine hydroxymethyltransferase, serine hydroxymethyltransferase 1, serine hydroxymethyltransferase 1 (soluble), serine hydroxymethyltransferase 1 (soluble) isoform 1, serine hydroxymethyltransferase 2 (mitochondrial), serine hydroxymethyltransferase, cytosolic, Serine methylase, SHMT, Shmt1, shmt1 protein, SHMT2, zgc:66171, zgc:77524, zSHMT-1
Host Species:
Rabbit
Purification Method:
Affinity Chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
20425, 287379, 6470
Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Concentration:
2.84 mg/mL
Formulation:
PBS with 50% glycerol and 0.01% thimerosal, pH 7.3
Gene Accession No.:
P34896, P50431
Gene Symbols:
Shmt1
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 285-444 of human SHMT1 (NP_6837181)
Quantity:
100 μL
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Rat
Product Type:
Antibody
Isotype:
IgG
Antigen: VAMP2
Classification: Polyclonal
Conjugate: Unconjugated
Gene: VAMP2
Gene Alias: RATVAMPB, RATVAMPIR, SYB, SYB2, Syb-2, sybII, Synaptobrevin, synaptobrevin 2, Synaptobrevin 2 (vesicle-associated membrane protein VAMP-2), synaptobrevin II, synaptobrevin-2, vamp, Vamp2, VAMP-2, vesicle associated membrane protein 2, vesicle-associated membrane 2, Vesicle-associated membrane protein (synaptobrevin 2), vesicle-associated membrane protein 2, vesicle-associated membrane protein 2 (synaptobrevin 2)
Host Species: Rabbit
Purification Method: Affinity Chromatography
Regulatory Status: RUO
Gene ID (Entrez): 22318, 24803, 6844
Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
Form: Liquid
Applications: Immunohistochemistry (Paraffin), Western Blot
Concentration: 0.42 mg/mL
Formulation: PBS with 50% glycerol and 0.01% thimerosal, pH 7.3
Gene Accession No.: P63027, P63044, P63045
Gene Symbols: VAMP2
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VAMP2 (NP_0550472)
Quantity: 100 μL
Primary or Secondary: Primary
Target Species: Human, Mouse, Rat
Product Type: Antibody
Isotype: IgG
Antigen:
VAMP2
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
VAMP2
Gene Alias:
RATVAMPB, RATVAMPIR, SYB, SYB2, Syb-2, sybII, Synaptobrevin, synaptobrevin 2, Synaptobrevin 2 (vesicle-associated membrane protein VAMP-2), synaptobrevin II, synaptobrevin-2, vamp, Vamp2, VAMP-2, vesicle associated membrane protein 2, vesicle-associated membrane 2, Vesicle-associated membrane protein (synaptobrevin 2), vesicle-associated membrane protein 2, vesicle-associated membrane protein 2 (synaptobrevin 2)
Host Species:
Rabbit
Purification Method:
Affinity Chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
22318, 24803, 6844
Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Applications:
Immunohistochemistry (Paraffin), Western Blot
Concentration:
0.42 mg/mL
Formulation:
PBS with 50% glycerol and 0.01% thimerosal, pH 7.3
Gene Accession No.:
P63027, P63044, P63045
Gene Symbols:
VAMP2
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VAMP2 (NP_0550472)
Quantity:
100 μL
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Rat
Product Type:
Antibody
Isotype:
IgG
Antigen: SIAH1
Classification: Polyclonal
Conjugate: Unconjugated
Gene: SIAH1
Gene Alias: AA960570, AA982064, AI853500, E3 ubiquitin-protein ligase SIAH1, E3 ubiquitin-protein ligase SIAH1A, E3 ubiquitin-protein ligase SIAH1B, FLJ08065, h HUMSIAH, hSIAH1, HUMSIAH, mSiah-1a, RING-type E3 ubiquitin transferase SIAH1, RING-type E3 ubiquitin transferase SIAH1A, RING-type E3 ubiquitin transferase SIAH1B, seven in absentia 1A, seven in absentia 1B, seven in absentia homolog 1, Seven in absentia homolog 1a, Seven in absentia homolog 1b, SIAH, siah E3 ubiquitin protein ligase 1, SIAH1, siah-1, Siah1a, Siah-1a, Siah1b, siah-1b, Sinh1a, Sinh1b
Host Species: Rabbit
Purification Method: Affinity Chromatography
Regulatory Status: RUO
Gene ID (Entrez): 110337437, 20437, 20438, 6477
Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
Form: Liquid
Applications: Western Blot
Concentration: 0.3 mg/mL
Formulation: PBS with 50% glycerol and 0.02% sodium azide, pH 7.3
Gene Accession No.: P61092, Q06985, Q8IUQ4
Gene Symbols: SIAH1, Siah1a, Siah1b
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SIAH1 (NP_0010066111)
Quantity: 100 μL
Primary or Secondary: Primary
Target Species: Human, Mouse
Product Type: Antibody
Isotype: IgG
Antigen:
SIAH1
Classification:
Polyclonal
Conjugate:
Unconjugated
Gene:
SIAH1
Gene Alias:
AA960570, AA982064, AI853500, E3 ubiquitin-protein ligase SIAH1, E3 ubiquitin-protein ligase SIAH1A, E3 ubiquitin-protein ligase SIAH1B, FLJ08065, h HUMSIAH, hSIAH1, HUMSIAH, mSiah-1a, RING-type E3 ubiquitin transferase SIAH1, RING-type E3 ubiquitin transferase SIAH1A, RING-type E3 ubiquitin transferase SIAH1B, seven in absentia 1A, seven in absentia 1B, seven in absentia homolog 1, Seven in absentia homolog 1a, Seven in absentia homolog 1b, SIAH, siah E3 ubiquitin protein ligase 1, SIAH1, siah-1, Siah1a, Siah-1a, Siah1b, siah-1b, Sinh1a, Sinh1b
Host Species:
Rabbit
Purification Method:
Affinity Chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
110337437, 20437, 20438, 6477
Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Applications:
Western Blot
Concentration:
0.3 mg/mL
Formulation:
PBS with 50% glycerol and 0.02% sodium azide, pH 7.3
Gene Accession No.:
P61092, Q06985, Q8IUQ4
Gene Symbols:
SIAH1, Siah1a, Siah1b
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SIAH1 (NP_0010066111)
Quantity:
100 μL
Primary or Secondary:
Primary
Target Species:
Human, Mouse
Product Type:
Antibody
Isotype:
IgG