Invitrogen™ BLOCK-iT™ Transfection Optimization Kit

Description

• The BLOCK-iT™ Transfection Optimization Kit is designed to optimize RNAi transfection using controls for transfection and viability
• Each kit contains a BLOCK-iT™ Fluorescent Oligo, which provides: A strong, easily detectable FITC signal indicating transfection efficiency Chemical modifications for a clearer, more persistent cellular signal than labeled siRNA provides Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA molecules This makes BLOCK-iT™ Fluorescent Oligo an ideal indicator of transfection efficiency for RNAi experiments
• In addition, the kit contains a Positive Control Stealth RNAi™ siRNA molecule directed at human p53 and a Scrambled Stealth RNAi™ siRNA molecule as a negative control
• To assay cell viability, Dead Cell Dye is included
• This dye colors dead cells as red, thereby allowing easy identification of non-viable cells in a tissue culture plate
• Using these reagents together allows you to both optimize transfection conditions and have a functional positive control in every RNAi experiment you perform.