Invitrogen™ Human Estrogen Receptor alpha Synthetic Peptide

Description

• ESR1 Synthetic Peptide, PEP-1620, from Invitrogen
• Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors
• The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA
• When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins
• There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively)
• Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa))
• Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function
• They also play a role in other tissues such as bone
• Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis
• This peptide corresponds to 18 amino acids near the carboxy terminus of ESR1.PEP-1620 can be used as a blocking peptide with polyclonal antibody PA5-34577
• For use as a blocking peptide in Western blot, incubate the peptide with equal volume of antibody for 30 min at 37°C.