Molecular Probes™ Vybrant™ DyeCycle™ Ruby Stain, 100 reactions

Description

• Vybrant™ DyeCycle™ Ruby Stain is a cell permeable DNA dye that can be used for cell cycle analysis on a 488 nm or 635 nm red diode laser
• Precise—accurate cell cycle analysis in living cells Safe—low cytotoxicity for cell sorting and additional live cell experiments Minimal compensation—easier multiplexing Simple, robust staining protocol View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry
• Precise Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability
• The Vybrant™ DyeCycle™ Ruby Stain is an ideal tool for DNA content analysis in living cells since the stain is cell permeable and, after binding double-stranded DNA, emits a fluorescent signal that is proportional to the DNA mass (see figure)
• Low Cytotoxicity Unlike stains that require high concentrations or have chemical structures that are toxic to cells, Vybrant™ DyeCycle™ Ruby Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle
• Minimal Compensation Well-suited for the popular red 633 nm laser line, the Vybrant™ DyeCycle™ Ruby Stain/DNA complex has fluorescence excitation and emission maxima of 638/686 nm, respectively (see figure)
• The spectral characteristics allow use of the red laser for cell cycle analysis and frees up other channels for additional parameters
• The red excitation and narrow emission of Vybrant™ DyeCycle™ Ruby Stain make it ideal for multiplexing due to the limited spectral overlap with other common dyes (Alexa Fluor™ 488, FITC, and RPE) and fluorescent proteins (Green Fluorescent Protein (GFP))
• Simple, Robust Staining Protocol For cell analysis, simply prepare flow cytometry tubes containing 0.5 mL of cells suspended in complete media at a concentration of 5 × 10 5 cells/mL
• To each tube, add 1μL of Vybrant™ DyeCycle™ Ruby Stain and mix well
• Final stain concentration is 5μM
• Incubate at 37°C for 15–30 minutes, protected from light
• Analyze without washing cells on a flow cytometer using 488 nm or 633/5 nm excitation and >670 nm emission.