Molecular Probes™ Vybrant™ DyeCycle™ Orange Stain

Description

• Vybrant™ DyeCycle™ Orange Stain is a cell permeable DNA dye that can be used for cell cycle analysis on 488 nm or 532 nm laser lines
• Precise—accurate cell cycle analysis in living cells Safe—low cytotoxicity for cell sorting and additional live cell experiments Simple, robust staining protocol View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry
• Precise Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability
• The Vybrant™ DyeCycle™ Orange Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure)
• Low Cytotoxicity Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant™ DyeCycle™ Orange Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle
• Simple, Robust Staining Protocol For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 10 6 cells/mL
• To each tube add 2μL of Vybrant™ DyeCycle™ Orange Stain
• Final stain concentration is 10μM
• Incubate at 37°C for 30 minutes, protected from light
• Analyze samples on flow cytometer using 488 nm excitation and orange emission.