Invitrogen™ Tali™ Apoptosis Kit - Annexin V Alexa Fluor™ 488 & Propidium Iodide

Description

• The TaliApoptosis Kit enables identification of apoptotic cells
• The kit stains apoptotic cells with green Annexin V – Alexa Fluor™ 488, stains necrotic cells with both red propidium iodide and green Annexin V – Alexa Fluor 488, and does not stain live cells
• Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development
• Inappropriately regulated apoptosis is implicated in disease states, such as Alzheimer's disease and cancer
• Apoptosis is distinguished from necrosis by characteristic morphological and biochemical changes, including compaction and fragmentation of the nuclear chromatin, shrinkage of the cytoplasm, and loss of membrane asymmetry
• Easily Discriminate Apoptotic from Necrotic Cells with Two-color Staining In normal live cells, phosphatidyl serine (PS) is located on the cytoplasmic surface of the cell membrane
• In apoptotic cells, however, PS is translocated from the inner to the outer surface of the plasma membrane, exposing it to the extracellular environment
• The human anticoagulant Annexin V displays a high affinity for PS
• Annexin V labeled with a fluorophore such as Alexa Fluor 488 can be used to identify apoptotic cells by binding to the exposed PS
• The TaliApoptosis Kit includes a solution of propidium Iodide (PI)
• PI is a cell-impermeant fluorogenic DNA-binding dye used for years to identify necrotic cells
• PI is impermeant to live cells, but easily enters dead cells where it binds to nucleic acids and becomes fluorescent
• After staining a cell population using the TaliApoptosis Assay Kit with Annexin V-Alexa Fluor 488 and Propidium Iodide, apoptotic cells display green fluorescence, dead cells display red and green fluorescence and live cells show little or no fluorescence
• Annexin V Translocation, Apoptosis, Cell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Viability, Proliferation and Function, Cellular Imaging, Imaging Cytometry Order Info Shipping Condition: Wet ice