A1460901
Gibco™ Angiogenesis Starter Kit
Manufacturer: Gibco™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A1460901-Each-of-1 | In Stock | ₹ 69,988.08 |
A1460901 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 69,988.08
GST (18%): ₹ 12,597.854
Total Price: ₹ 82,585.934
Cell Type
Endothelial Cells (Umbilical Vein)
Content And Storage
1 bottle M200500 MediumStore at 2–8°C1 bottle LVESStore at 2–8°C1 vial HUCEVStore in liquid nitrogen1 vial Geltrex LDEV-Free MatrixStore at -20 to -80°C
For Use With (Application)
Angiogenesis
Format
Cell Culture Plate (Multi-Well)
Quantity
1 kit
Color
Green
Detection Method
Fluorescence
For Use With (Equipment)
Microscope
Label or Dye
Calcein
Type
Angiogenesis Starter Kit
Related Products
Description
- The Gibco™ Angiogenesis Starter Kit contains the reagents and protocols necessary to perform various angiogenesis assays
- Designed to address the needs of both the occasional user as well as the most experienced researcher, this combination of high quality products is easy to use and cost effective
- Detailed protocols for the tube formation assay and cell migration are available below
- What’s Included: Gibco™ Large Vessel Endothelial Supplement (LVES), 50X, 11 ml, ready to use Gibco™ Medium 200 basal media, 500 ml Gibco™ Human Umbilical Vein Endothelial Cells (HUVEC), 1 vial, 5 x 10 5 cells Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix, 5 ml Large Vessel Endothelial Supplement and Medium 200 Gibco™ LVES is a 50X, complete, ready-to-use media supplement optimized for use with Gibco™ Medium 200
- This blended supplement has been designed to deliver optimal cell growth and to enable superior cell performance for angiogenesis applications such as cell migration and tube formation
- LVES contains fetal bovine serum, hydrocortisone, human epidermal growth factor, basic fibroblast growth factor, heparin, and ascorbic acid
- Human Umbilical Vein Endothelial Cells Gibco™ Human Umbilical Vein Endothelial Cells are cryopreserved at the end of the primary culture
- Upon thawing, they are guaranteed to be >70% viable and capable of a minimum of 16 population doublings
- Cells are derived from tissue obtained from accredited institutions with IRB-approved donor programs
- Quality control of these cells includes testing for mycoplasma, sterility, endotoxin, hepatitis B and C, and HIV
- Additionally, the cells are positive for von Willebrand factor (vWf), positive for CD31, negative for a-actin, and positive for dil-Ac-LDL uptake
- Geltrex™ LDEV-Free Matrix Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble, reduced growth factor, lactate dehydrogenase-elevating virus-free basement membrane extract used in a wide variety of cell culture applications for promotion and maintenance of many cell types, including primary epithelial cells, endothelial cells, and stem cells
- It can be diluted for use as a coating matrix for culture vessels or used undiluted as a thick gel preparation to create a more physiologically relevant environment for angiogenesis assays.
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Cell Type: Endothelial Cells (Umbilical Vein)
Content And Storage: 1 bottle M200500 MediumStore at 2–8°C1 bottle LVESStore at 2–8°C1 vial HUCEVStore in liquid nitrogen1 vial Geltrex LDEV-Free MatrixStore at -20 to -80°C
For Use With (Application): Angiogenesis
Format: Cell Culture Plate (Multi-Well)
Quantity: 1 kit
Color: Green
Detection Method: Fluorescence
For Use With (Equipment): Microscope
Label or Dye: Calcein
Type: Angiogenesis Starter Kit
Cell Type:
Endothelial Cells (Umbilical Vein)
Content And Storage:
1 bottle M200500 MediumStore at 2–8°C1 bottle LVESStore at 2–8°C1 vial HUCEVStore in liquid nitrogen1 vial Geltrex LDEV-Free MatrixStore at -20 to -80°C
For Use With (Application):
Angiogenesis
Format:
Cell Culture Plate (Multi-Well)
Quantity:
1 kit
Color:
Green
Detection Method:
Fluorescence
For Use With (Equipment):
Microscope
Label or Dye:
Calcein
Type:
Angiogenesis Starter Kit
Cell Type: 293-derived Cells
Content And Storage: 2 vials frozen Expi293F cells1 bottle 1000 mL Expi293 Expression Medium1 ExpiFectamine 293 transfection kit for 1 L of culture1 vial Antibody Expressing Positive Control Vector1 bottle 100 mL Opti-MEM Reduced Serum MediumStore all components at +2°C to +8°C, except frozen cells are stored in liquid nitrogen
For Use With (Application): Protein Expression
Format: __
Quantity: 1 kit
Color: __
Detection Method: __
For Use With (Equipment): __
Label or Dye: __
Type: __
Cell Type:
293-derived Cells
Content And Storage:
2 vials frozen Expi293F cells1 bottle 1000 mL Expi293 Expression Medium1 ExpiFectamine 293 transfection kit for 1 L of culture1 vial Antibody Expressing Positive Control Vector1 bottle 100 mL Opti-MEM Reduced Serum MediumStore all components at +2°C to +8°C, except frozen cells are stored in liquid nitrogen
For Use With (Application):
Protein Expression
Format:
__
Quantity:
1 kit
Color:
__
Detection Method:
__
For Use With (Equipment):
__
Label or Dye:
__
Type:
__
Cell Type: __
Content And Storage: 150 μg at 1 mg/mLStore at 2-8°C or long term storage at -20°C.
For Use With (Application): Protein Production, Constitutive Expression
Format: __
Quantity: 5 Reactions
Color: __
Detection Method: __
For Use With (Equipment): __
Label or Dye: __
Type: __
Cell Type:
__
Content And Storage:
150 μg at 1 mg/mLStore at 2-8°C or long term storage at -20°C.
For Use With (Application):
Protein Production, Constitutive Expression
Format:
__
Quantity:
5 Reactions
Color:
__
Detection Method:
__
For Use With (Equipment):
__
Label or Dye:
__
Type:
__
Cell Type: __
Content And Storage: 3 DNA vectors mixed into 1 tube at 1 μg/μl in TE buffer
For Use With (Application): Episomal Expression, Induced Pluripotent Stem Cell Generation, Multi-Gene Expression
Format: __
Quantity: 1 tube
Color: __
Detection Method: __
For Use With (Equipment): Electroporator, Neon™ Transfection System
Label or Dye: __
Type: __
Cell Type:
__
Content And Storage:
3 DNA vectors mixed into 1 tube at 1 μg/μl in TE buffer
For Use With (Application):
Episomal Expression, Induced Pluripotent Stem Cell Generation, Multi-Gene Expression
Format:
__
Quantity:
1 tube
Color:
__
Detection Method:
__
For Use With (Equipment):
Electroporator, Neon™ Transfection System
Label or Dye:
__
Type:
__