Invitrogen™ Episomal iPSC Reprogramming Vectors

Description

• Episomal iPSC Reprogramming Vectors are a cost-effective mixture of three vectors designed to provide the optimal system for generating transgene-free and virus-free induced pluripotent stem cells (iPSCs) in a feeder-free environment
• Originally developed by Junying Yu and James Thomson(1) and further optimized by Cellular Dynamics International, these Episomal iPSC Reprogramming Vectors have proven successful in reprogramming a number of different somatic cell types
• Safe for all stages of your iPSC research—transgene-free and viral-free reprogramming allows use from basic to pre-clinical research Reprogram a variety of somatic cell types—provides flexibility in somatic cell selection Optimized for feeder-free reprogramming—allows for defined and feeder-free reprogramming when used with Essential 8™ Medium Create Transgene- and Virus-free iPSCs The Episomal iPSC Reprogramming Vectors are a well-described system for producing transgene-free, virus-free iPSCs, providing a source of iPSCs for all stages of your pluripotent stem cell research
• Other reprogramming methods, such as lentivirus, contain transgenes that can integrate into the host genome, potentially disrupting the genome or causing unpredictable results
• These episomal vectors are introduced into the cell by electroporation
• As oriP/EBNA1 vectors, they contain all 6 reprogramming factors (Oct4, Sox2, Nanog, Lin28, Klf4 and lMyc) and replicate extrachromosomally only once per cell cycle
• At this replication rate, the episomes are lost at a rate of approximately 5% per cell generation
• Generate iPSCs from a Wide Variety of Somatic Cell Types iPSCs have been generated with episomal vectors from a range of somatic cells including fibroblasts, bone marrow mononuclear cells, PBMCs, lymphoblast B cells, and various disease-type fibroblasts and PBMCs
• Each kit provides enough material for 6 reprogramming experiments
• Optimized for Feeder-free Reprogramming with Essential 8™ Medium The Episomal iPSC Reprogramming Vectors were designed in the laboratories of James Thomson and Cellular Dynamics International for use with Essential 8™ Medium, thus providing an optimal environment for defined, feeder-free reprogramming
• Commercialized in Partnership with Cellular Dynamics International
• For Research Use Only
• Not intended for any animal or human therapeutic or diagnostic use
• Reference: 1
• Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences; Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson J
• Science
• 2009; 324:797-801.