A15004
pJTI™ R4 CMV-TO MCS pA Vector
Manufacturer: LIFE TECHNOLOGIES
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A15004-Each-of-1 | In Stock | ₹ 1,27,655.52 |
A15004 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,27,655.52
GST (18%): ₹ 22,977.994
Total Price: ₹ 1,50,633.514
Constitutive or Inducible System
Inducible
Inducing Agent
Tetracycline
Product Type
Mammalian Expression Vector
Content And Storage
pJTI™ R4 CMV-TO MCS pA vector (100 μg), store at -20 °C
Protein Tag
Untagged
Vector
Jump-In Vectors
For Use With (Application)
Stable Cell Line Development, Targeted Integration, Regulated Expression
Delivery Type
Transfection
Promoter
CMV/TO
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Cloning Method
Restriction Enzyme/MCS
Product Line
Jump-In™
Related Products
Description
- The pJTI™ R4 CMV-TO MCS pA vector is designed for the expression of your gene of interest under the control of the tet-inducible CMV promoter after restriction enzyme cloning and retargeting into the genomic R4 site of a Jump-In™ parental cell line
- This vector can be used for inducible or constitutive expression of your gene of interest, depending on which Jump-In™ parental cell line you use
- When this vector is retargeted into a Jump-In™ T-REx™ parental cell line , gene expression is controlled by the tet-operon and can be induced by adding doxycycline to the growth media
- When used with a Jump-In™ parental cell line such as the Jump-In™ GripTite™ HEK293 cell line , constitutive expression is achieved after retargeting
- The R4 sites in the Jump-In™ parental cell lines result in a high retargeting efficiency, requiring less effort and less time than traditional cell engineering methods
- Retargeting of Jump-In™ parental cell lines results in creation of an isogenic pool that is sufficient for cell-based experiments without the need for clonal selection
- Alternatively, the high retargeting efficiency allows for easy selection of a positive stable clone for expressing your gene of interest.
Compare Similar Items
Show Difference
Constitutive or Inducible System: Inducible
Inducing Agent: Tetracycline
Product Type: Mammalian Expression Vector
Content And Storage: pJTI™ R4 CMV-TO MCS pA vector (100 μg), store at -20 °C
Protein Tag: Untagged
Vector: Jump-In Vectors
For Use With (Application): Stable Cell Line Development, Targeted Integration, Regulated Expression
Delivery Type: Transfection
Promoter: CMV/TO
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Cloning Method: Restriction Enzyme/MCS
Product Line: Jump-In™
Constitutive or Inducible System:
Inducible
Inducing Agent:
Tetracycline
Product Type:
Mammalian Expression Vector
Content And Storage:
pJTI™ R4 CMV-TO MCS pA vector (100 μg), store at -20 °C
Protein Tag:
Untagged
Vector:
Jump-In Vectors
For Use With (Application):
Stable Cell Line Development, Targeted Integration, Regulated Expression
Delivery Type:
Transfection
Promoter:
CMV/TO
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Antibiotic Resistance Bacterial:
Ampicillin (AmpR)
Cloning Method:
Restriction Enzyme/MCS
Product Line:
Jump-In™
Constitutive or Inducible System: Inducible
Inducing Agent: Tetracycline
Product Type: Mammalian Expression Vector
Content And Storage: pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 μg), store at -20 °C
Protein Tag: GFP (EmGFP)
Vector: Jump-In Vectors
For Use With (Application): Stable Cell Line Development, Targeted Integration, Regulated Expression
Delivery Type: Transfection
Promoter: CMV/TO
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Cloning Method: __
Product Line: Jump-In™
Constitutive or Inducible System:
Inducible
Inducing Agent:
Tetracycline
Product Type:
Mammalian Expression Vector
Content And Storage:
pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 μg), store at -20 °C
Protein Tag:
GFP (EmGFP)
Vector:
Jump-In Vectors
For Use With (Application):
Stable Cell Line Development, Targeted Integration, Regulated Expression
Delivery Type:
Transfection
Promoter:
CMV/TO
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Antibiotic Resistance Bacterial:
Ampicillin (AmpR)
Cloning Method:
__
Product Line:
Jump-In™
Constitutive or Inducible System: __
Inducing Agent: Tetracycline
Product Type: CHO-K1 Kit
Content And Storage: • Jump-In™ T-REx™ CHO-K1 Cells, 2 x 1 mL vials (store in liquid nitrogen)• pJTI™ R4 DEST CMV TO pA vector, 100 μg (store at -20°C)• pJTI™ R4 Int vector, 100 μg (store at -20°C)
Protein Tag: __
Vector: pJTI™ R4 DEST CMV TO pA , pJTI™ R4 Int
For Use With (Application): Assay development
Delivery Type: Transfection
Promoter: CMV/TO
Selection Agent (Eukaryotic): Geneticin™ (G-418), Hygromycin, Blasticidin
Antibiotic Resistance Bacterial: __
Cloning Method: __
Product Line: Jump-In™
Constitutive or Inducible System:
__
Inducing Agent:
Tetracycline
Product Type:
CHO-K1 Kit
Content And Storage:
• Jump-In™ T-REx™ CHO-K1 Cells, 2 x 1 mL vials (store in liquid nitrogen)• pJTI™ R4 DEST CMV TO pA vector, 100 μg (store at -20°C)• pJTI™ R4 Int vector, 100 μg (store at -20°C)
Protein Tag:
__
Vector:
pJTI™ R4 DEST CMV TO pA , pJTI™ R4 Int
For Use With (Application):
Assay development
Delivery Type:
Transfection
Promoter:
CMV/TO
Selection Agent (Eukaryotic):
Geneticin™ (G-418), Hygromycin, Blasticidin
Antibiotic Resistance Bacterial:
__
Cloning Method:
__
Product Line:
Jump-In™
Constitutive or Inducible System: __
Inducing Agent: Tetracycline
Product Type: HEK293 Kit
Content And Storage: • Jump-In™ T-REx™ HEK293 Cells, 2 x 1 mL vials (store in liquid nitrogen)• pJTI™ R4 DEST CMV TO pA vector, 100 μg (store at -20°C)• pJTI™ R4 Int vector, 100 μg (store at -20°C)
Protein Tag: __
Vector: pJTI™ R4 DEST CMV TO pA , pJTI™ R4 Int
For Use With (Application): Protein expression, Assay development
Delivery Type: Transfection
Promoter: CMV/TO
Selection Agent (Eukaryotic): Geneticin™ (G-418), Hygromycin, Blasticidin
Antibiotic Resistance Bacterial: __
Cloning Method: __
Product Line: Jump-In™
Constitutive or Inducible System:
__
Inducing Agent:
Tetracycline
Product Type:
HEK293 Kit
Content And Storage:
• Jump-In™ T-REx™ HEK293 Cells, 2 x 1 mL vials (store in liquid nitrogen)• pJTI™ R4 DEST CMV TO pA vector, 100 μg (store at -20°C)• pJTI™ R4 Int vector, 100 μg (store at -20°C)
Protein Tag:
__
Vector:
pJTI™ R4 DEST CMV TO pA , pJTI™ R4 Int
For Use With (Application):
Protein expression, Assay development
Delivery Type:
Transfection
Promoter:
CMV/TO
Selection Agent (Eukaryotic):
Geneticin™ (G-418), Hygromycin, Blasticidin
Antibiotic Resistance Bacterial:
__
Cloning Method:
__
Product Line:
Jump-In™