Invitrogen™ pcDNA™4/TO Mammalian Expression Vector

Description

• Uses repressor mechanism that blocks transcription from the powerful CMV promoter in absence of tetracycline Because the T-REx System elements do not use viral transactivators, user can achieve high-level expression from complete CMV promoter without secondary, nonspecific activation of host genes Two tetracycline operator sequences (TetO 2 ) have been inserted between TATA box of the CMV promoter and the transcriptional start site: The TetO 2 sequence itself has no effect on expression When tetracycline repressor protein (TR) is present, it effectively binds TetO 2 sites and blocks transcription initiation Tetracycline added to culture medium binds to, and changes conformation of, TR protein This change causes TR protein to release TetO 2 sites, derepressing transcription from the CMV promoter for high-level expression of gene of interest Expression levels can be modulated based on tetracycline concentration and can be induced to levels that are achieved with constitutive CMV expression vectors Zeocin or hygromycin resistance gene for effective selection of stable mammalian cell lines Large multiple cloning site to simplify cloning Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Regulated Expression, Targeted Integration