A15005
pJTI™ R4 EXP CMV-TO EmGFP pA Vector
Manufacturer: LIFE TECHNOLOGIES
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A15005-Each-of-1 | In Stock | ₹ 25,496.88 |
A15005 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 25,496.88
GST (18%): ₹ 4,589.438
Total Price: ₹ 30,086.318
Constitutive or Inducible System
Inducible
Inducing Agent
Tetracycline
Product Type
Mammalian Expression Vector
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Quantity
100 μg
Product Line
Jump-In™
Delivery Type
Transfection
Promoter
CMV/TO
Reporter Gene
GFP (EmGFP)
Content And Storage
pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 μg), store at -20 °C
Protein Tag
GFP (EmGFP)
Vector
Jump-In Vectors
For Use With (Application)
Stable Cell Line Development, Targeted Integration, Regulated Expression
Related Products
Description
- The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line
- When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection, the EmGFP can be inducibly expressed with doxycycline and the successfully retargeted cells will fluoresce green
- Ensure the Success of Your Jump-In™ T-REx™ Retargeting Reactions Successful retargeting of Jump-In™ T-REx™ parental cell lines like the Jump-In™ T-REx™ HEK293 Kit is dependent on a variety of factors, such as: Transfection efficiency Cell confluency Antibiotic selection conditions Quality and concentration of DNA Retargeting vector to integrase vector ratio We strongly recommend including a positive control retargeting reaction using the pJTI™ R4 Exp CMV-TO EmGFP pA vector in your Jump-In™ experiment, along with negative controls (no plasmid DNA, no integrase vector), so you can easily visualize the results and optimize the retargeting conditions.
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Constitutive or Inducible System: Inducible
Inducing Agent: Tetracycline
Product Type: Mammalian Expression Vector
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Quantity: 100 μg
Product Line: Jump-In™
Delivery Type: Transfection
Promoter: CMV/TO
Reporter Gene: GFP (EmGFP)
Content And Storage: pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 μg), store at -20 °C
Protein Tag: GFP (EmGFP)
Vector: Jump-In Vectors
For Use With (Application): Stable Cell Line Development, Targeted Integration, Regulated Expression
Constitutive or Inducible System:
Inducible
Inducing Agent:
Tetracycline
Product Type:
Mammalian Expression Vector
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Antibiotic Resistance Bacterial:
Ampicillin (AmpR)
Quantity:
100 μg
Product Line:
Jump-In™
Delivery Type:
Transfection
Promoter:
CMV/TO
Reporter Gene:
GFP (EmGFP)
Content And Storage:
pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 μg), store at -20 °C
Protein Tag:
GFP (EmGFP)
Vector:
Jump-In Vectors
For Use With (Application):
Stable Cell Line Development, Targeted Integration, Regulated Expression
Constitutive or Inducible System: __
Inducing Agent: Tetracycline
Product Type: CHO-K1 Kit
Selection Agent (Eukaryotic): Geneticin™ (G-418), Hygromycin, Blasticidin
Antibiotic Resistance Bacterial: __
Quantity: 1 Kit
Product Line: Jump-In™
Delivery Type: Transfection
Promoter: CMV/TO
Reporter Gene: __
Content And Storage: • Jump-In™ T-REx™ CHO-K1 Cells, 2 x 1 mL vials (store in liquid nitrogen)• pJTI™ R4 DEST CMV TO pA vector, 100 μg (store at -20°C)• pJTI™ R4 Int vector, 100 μg (store at -20°C)
Protein Tag: __
Vector: pJTI™ R4 DEST CMV TO pA , pJTI™ R4 Int
For Use With (Application): Assay development
Constitutive or Inducible System:
__
Inducing Agent:
Tetracycline
Product Type:
CHO-K1 Kit
Selection Agent (Eukaryotic):
Geneticin™ (G-418), Hygromycin, Blasticidin
Antibiotic Resistance Bacterial:
__
Quantity:
1 Kit
Product Line:
Jump-In™
Delivery Type:
Transfection
Promoter:
CMV/TO
Reporter Gene:
__
Content And Storage:
• Jump-In™ T-REx™ CHO-K1 Cells, 2 x 1 mL vials (store in liquid nitrogen)• pJTI™ R4 DEST CMV TO pA vector, 100 μg (store at -20°C)• pJTI™ R4 Int vector, 100 μg (store at -20°C)
Protein Tag:
__
Vector:
pJTI™ R4 DEST CMV TO pA , pJTI™ R4 Int
For Use With (Application):
Assay development
Constitutive or Inducible System: __
Inducing Agent: Tetracycline
Product Type: HEK293 Kit
Selection Agent (Eukaryotic): Geneticin™ (G-418), Hygromycin, Blasticidin
Antibiotic Resistance Bacterial: __
Quantity: 1 Kit
Product Line: Jump-In™
Delivery Type: Transfection
Promoter: CMV/TO
Reporter Gene: __
Content And Storage: • Jump-In™ T-REx™ HEK293 Cells, 2 x 1 mL vials (store in liquid nitrogen)• pJTI™ R4 DEST CMV TO pA vector, 100 μg (store at -20°C)• pJTI™ R4 Int vector, 100 μg (store at -20°C)
Protein Tag: __
Vector: pJTI™ R4 DEST CMV TO pA , pJTI™ R4 Int
For Use With (Application): Protein expression, Assay development
Constitutive or Inducible System:
__
Inducing Agent:
Tetracycline
Product Type:
HEK293 Kit
Selection Agent (Eukaryotic):
Geneticin™ (G-418), Hygromycin, Blasticidin
Antibiotic Resistance Bacterial:
__
Quantity:
1 Kit
Product Line:
Jump-In™
Delivery Type:
Transfection
Promoter:
CMV/TO
Reporter Gene:
__
Content And Storage:
• Jump-In™ T-REx™ HEK293 Cells, 2 x 1 mL vials (store in liquid nitrogen)• pJTI™ R4 DEST CMV TO pA vector, 100 μg (store at -20°C)• pJTI™ R4 Int vector, 100 μg (store at -20°C)
Protein Tag:
__
Vector:
pJTI™ R4 DEST CMV TO pA , pJTI™ R4 Int
For Use With (Application):
Protein expression, Assay development
Constitutive or Inducible System: __
Inducing Agent: __
Product Type: __
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial: __
Quantity: __
Product Line: __
Delivery Type: __
Promoter: __
Reporter Gene: __
Content And Storage: __
Protein Tag: __
Vector: __
For Use With (Application): __
Constitutive or Inducible System:
__
Inducing Agent:
__
Product Type:
__
Selection Agent (Eukaryotic):
__
Antibiotic Resistance Bacterial:
__
Quantity:
__
Product Line:
__
Delivery Type:
__
Promoter:
__
Reporter Gene:
__
Content And Storage:
__
Protein Tag:
__
Vector:
__
For Use With (Application):
__