Invitrogen™ BacMam GFP Transduction Control (BacMam 2.0)

Description

• The BacMam 2.0 Transduction Control lets you experience the power and convenience of enhanced BacMam technology, with the convenient readout of emerald GFP (emGFP); our Aequorea victoria emGFP is extremely bright and photostable and this non-targeted form is ideal for testing transduction
• Check out how well BacMam 2.0 works with GFP in your model before advancing to the more specialized reagents
• The BacMam technology is based on an insect virus (baculovirus) to help efficiently deliver and express genes in mammalian cells
• The baculovirus has been modified to include an expression cassette for transgene expression in mammalian cells
• BacMam 2.0 incorporate elements that help greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry and genetic elements (enhanced CMV promoter and Woodchuck Post-transcriptional Regulatory Element), that boost expression levels
• Baculoviruses do not replicate in mammalian cells and thus have an excellent safety profile and lack cytopathic effects on cells
• Cell types previously not compatible with the technology (neuronal cells, T-cells), or only poorly transduced (some stem cells, CHO) are now transduced quantitatively in a simple, one step process
• Efficient : highly efficient (>90%) transduction of a wide range of mammalian cell lines, including primary cells, stem cells and neuronal cells Fast and convenient : Simply add the BacMam 2.0 together with cells to your cover slip or microtiter plate, incubate overnight and assay or store frozen, assay-ready cells for later use Safe : non-replicating in mammalian cells, lack of observable cytopathic effect and biosafety level (BSL) 1 handling Enables Flexiblity : co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies and tightly control expression levels by just varying the dose Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Fluorescent Protein Assays (GFP) Order Info Shipping Condition: Wet ice