Molecular Probes™ CyQUANT™ Cell Proliferation Assay, for cells in culture

Description

• The CyQUANT™ Cell Proliferation Assay is a highly sensitive fluorescence-based method for quantifying cells and assessing cell proliferation and cytotoxicity
• For cell proliferation assays that are more compatible with high throughput screening, see our CyQUANT™ NF Cell Proliferation Assay Kit or our CyQUANT™ Direct Assay
• With the CyQUANT™ Cell Proliferation Assay Kit, you can: Measure DNA content to directly quantify 50 to 50,000 cells per well without relying on metabolic activity Quickly compare multiple samples in a simple plate-based assay format Freeze and store time course samples for later analysis Measure DNA content directly The main component of the CyQUANT™ Cell Proliferation Assay Kit is CyQUANT™ GR, a proprietary dye that exhibits strong fluorescence enhancement when bound to nucleic acids
• With this dye, you can measure a sample's DNA content and compare it to standards, directly quantifying the entire cell population within a broad linear detection range
• This method offers improved accuracy over metabolically based cell proliferation or cytotoxicity assays that can be influenced by cell changes that are unrelated to cell number
• Quickly compare multiple samples The CyQUANT™ Cell Proliferation Assay Kit requires minimal hands-on time
• Simply remove the culture media, freeze and lyse cells, then read fluorescence on a standard microplate reader with a fluorescein filter
• Store samples for later analysis Because the cells are frozen, samples can stored for up to 4 weeks and run in batches, allowing the direct comparison of samples taken at different time points
• Kit components The kit includes CyQUANT™ GR dye and cell lysis buffer sufficient to perform 1,000 assays in volumes suitable for fluorescence detection in microplates
• The kit also contains bacteriophage λ DNA to be used as a reference standard for assay calibration.