Invitrogen™ Image-iT™ Cell Painting Kit

Description

• The Image-iT Cell Painting Kit conveniently provides six different fluorescent labels to assess cellular morphology by high content screening for drug discovery
• Cell painting is useful for identifying cellular phenotypic changes, mechanisms of action, toxicity testing, and cellular profiling during high-content drug screening
• This image-based assay known as cell painting uses fluorescent multiplexing to paint the specific organelle with its own label within the cell
• Using different fluorophores, the Image-iT Cell Painting Kit allows for cellular image-based profiling to characterize a cell’s response while visualizing and analyzing the spatial organization of structures to assess any morphological changes after exposure to drug candidates and other perturbations
• In addition, cell painting allows researchers to investigate the organization of proteins, cell viability, and proliferation or visualize DNA damage
• Cytological profiling studies can be performed using cell painting to evaluate the effects of compounds on cellular morphology to help identify drug candidates in high content screening assays
• When running high-content screening and imaging assays, high image quality is essential
• Several factors can impair image quality, such as using thick, uneven plates, which can lead to focusing differences between wells and image quality issues across a plate
• We recommend using our Optical-Quality Imaging Plates (Cat
• No
• A58941) for superior image quality and resolution when used with fluorescence or luminescence readers
• When combined with the CellInsight High Content Screening (HCS) Platform, these cell painting kit reagents provide a phenotypical readout to depict cell health
• The kit is based on the publication by Bray et al
• (2016) that identified the morphological profiling of cells using multiplexed fluorescent dyes
• Image-iT Cell Painting kits can be purchased with enough reagents for either two or ten 384-well plates
• This kit includes the following reagents: • Hoechst 34580, trihydrochloride, trihydrate • Concanavalin A, Alexa Fluor 488 conjugate • Wheat Germ Agglutinin, Alexa Fluor 555 conjugate • Alexa Fluor 568 Phalloidin • SYTO 14 Green Fluorescent Nucleic Acid Stain • MitoTracker Deep Red FM