K1032
Invitrogen™ LiveBLAzer™ FRET-B/G Loading Kit with CCF2-AM
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1032-Each-of-1 | In Stock | ₹ 1,14,137.04 |
K1032 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,14,137.04
GST (18%): ₹ 20,544.667
Total Price: ₹ 1,34,681.707
Cell Permeability
Cell-Permeant
Detection Method
Fluorescence
Product Line
LiveBLAzer™
Substrate Properties
Chemical Substrate
Substrate
CCF2-AM
Content And Storage
GeneBLAzer™ beta-lactamase substrates are supplied as loading kits (Table 1) and are available in bulk sizes. Loading kits include CCF2-AM or CCF4-AM, DMSO (for Solution A), Solution B, and Solution C.CCF2-AM and CCF4-AM are provided dry, and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A), Solution B, and Solution C should be stored at 22°C to 25°C, protected from direct light. Slightly cooler temperatures may cause a white precipitate to form in Solution B.
For Use With (Equipment)
Flow Cytometer, Microplate Reader
Quantity
200 μg
Substrate Type
Beta-Lactamase Substrate
Type
FRET-B/G Loading Kit
Related Products
Description
- GeneBLAzer™ cell-based assays utilize the membrane-permeant ester forms (CCF2-AM and CCF4-AM) of the negatively charged fluorescent beta-lactamase substrates, CCF2 and CCF4
- These lipophilic esters readily enter the cell, where cleavage by endogeneous cytoplasmic esterases rapidly converts them into their negatively charged forms, thereby trapping them in the cytosol
- Detection of GeneBLAzer™ assays is FRET-based
- Each substrate is labeled with two fluorophores that form an efficient FRET pair
- In the absence of beta-lactamase activity, exciting the coumarin at 409 nm in the intact CCF2 molecule results in FRET to the fluorescein, which emits a green fluorescence signal at 518 nm (Figure 1)
- In the presence of beta-lactamase activity, however, cleavage of CCF2 spatially separates the two dyes and disrupts FRET, so that exciting the coumarin at 409 nm now produces a blue fluorescence signal at 447 nm
- This blue signal can be readily observed under a microscope and can also be detected as an increase in the blue channel readout on fluorescent microplate readers
- The CCF2-AM and CCF4-AM substrates are essential assay components for the GeneBLAzer™ platform
- These substrates are fully compatible with flow cytometry, speeding the time to clone selection
- Ratiometric analysis of the blue and green signals reduces well-to-well variation due to differences in cell numbers and substrate loading, leading to high Z'-factor values and low coefficients of variation (CVs)
- CCF2-AM and CCF4-AM differ by two carbons in the bridge linking the coumarin moiety to the lactam ring
- Both are in the membrane-permeable, esterified forms, and can be used for assays in intact cells
- CCF4-AM has better solubility properties (soluble for >24 hours) than CCF2-AM and is thus best suited for screening applications
- In addition, CCF4-AM has slightly better FRET and thus slightly lower background than CCF2-AM
- CCF2-FA is essentially the CCF2 substrate without the esters found in the AM version
- CCF2-FA is de-esterified and used in cell lysate applications, bypassing loading across the cell membrane and de-esterification steps
- Cell lysates are the preferred method for applications using cells that contain a cell wall
- CCF2-FA can also be used as a control to acquire the excitation and emission spectra for CCF2-AM and CCF4-AM.
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Cell Permeability: Cell-Permeant
Detection Method: Fluorescence
Product Line: LiveBLAzer™
Substrate Properties: Chemical Substrate
Substrate: CCF2-AM
Content And Storage: GeneBLAzer™ beta-lactamase substrates are supplied as loading kits (Table 1) and are available in bulk sizes. Loading kits include CCF2-AM or CCF4-AM, DMSO (for Solution A), Solution B, and Solution C.CCF2-AM and CCF4-AM are provided dry, and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A), Solution B, and Solution C should be stored at 22°C to 25°C, protected from direct light. Slightly cooler temperatures may cause a white precipitate to form in Solution B.
For Use With (Equipment): Flow Cytometer, Microplate Reader
Quantity: 200 μg
Substrate Type: Beta-Lactamase Substrate
Type: FRET-B/G Loading Kit
Cell Permeability:
Cell-Permeant
Detection Method:
Fluorescence
Product Line:
LiveBLAzer™
Substrate Properties:
Chemical Substrate
Substrate:
CCF2-AM
Content And Storage:
GeneBLAzer™ beta-lactamase substrates are supplied as loading kits (Table 1) and are available in bulk sizes. Loading kits include CCF2-AM or CCF4-AM, DMSO (for Solution A), Solution B, and Solution C.CCF2-AM and CCF4-AM are provided dry, and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A), Solution B, and Solution C should be stored at 22°C to 25°C, protected from direct light. Slightly cooler temperatures may cause a white precipitate to form in Solution B.
For Use With (Equipment):
Flow Cytometer, Microplate Reader
Quantity:
200 μg
Substrate Type:
Beta-Lactamase Substrate
Type:
FRET-B/G Loading Kit
Cell Permeability: Cell-Impermeant
Detection Method: Fluorescence
Product Line: GeneBLAzer™
Substrate Properties: Chemical Substrate
Substrate: CCF2-FA
Content And Storage: Store in freezer (-5 to -30°C).
For Use With (Equipment): __
Quantity: 100μg
Substrate Type: Beta-Lactamase Substrate
Type: __
Cell Permeability:
Cell-Impermeant
Detection Method:
Fluorescence
Product Line:
GeneBLAzer™
Substrate Properties:
Chemical Substrate
Substrate:
CCF2-FA
Content And Storage:
Store in freezer (-5 to -30°C).
For Use With (Equipment):
__
Quantity:
100μg
Substrate Type:
Beta-Lactamase Substrate
Type:
__
Cell Permeability: __
Detection Method: Fluorescence
Product Line: GeneBLAzer™
Substrate Properties: __
Substrate: __
Content And Storage: GeneBLAzer™ CMV-blaJurkat Positive Control Cell Line is shipped on dry ice and should be stored in liquid nitrogen.
For Use With (Equipment): __
Quantity: 1 vial
Substrate Type: __
Type: Cell Line
Cell Permeability:
__
Detection Method:
Fluorescence
Product Line:
GeneBLAzer™
Substrate Properties:
__
Substrate:
__
Content And Storage:
GeneBLAzer™ CMV-blaJurkat Positive Control Cell Line is shipped on dry ice and should be stored in liquid nitrogen.
For Use With (Equipment):
__
Quantity:
1 vial
Substrate Type:
__
Type:
Cell Line
Cell Permeability: __
Detection Method: __
Product Line: GeneSwitch™
Substrate Properties: __
Substrate: __
Content And Storage: The GeneSwitch™ System Core Kit includes 20 μg of each of the following vectors: pGene/V5-His (A, B, and C), the expression control vector pGene/V5-His/lacZ, and the pSwitch vector. The kit also includes forward and reverse sequencing primers and 100 μg of the inducing agent mifepristone. The GeneSwitch™ Complete Kit includes the Core Kit with 1 g hygromycin and 1 g Zeocin™ at considerable savings. Hygromycin should be stored at +4°C and Zeocin™ at -20°C. Zeocin™ should be protected from exposure to light. All reagents are stable for 6 months when properly stored.
For Use With (Equipment): __
Quantity: 1 kit
Substrate Type: __
Type: __
Cell Permeability:
__
Detection Method:
__
Product Line:
GeneSwitch™
Substrate Properties:
__
Substrate:
__
Content And Storage:
The GeneSwitch™ System Core Kit includes 20 μg of each of the following vectors: pGene/V5-His (A, B, and C), the expression control vector pGene/V5-His/lacZ, and the pSwitch vector. The kit also includes forward and reverse sequencing primers and 100 μg of the inducing agent mifepristone. The GeneSwitch™ Complete Kit includes the Core Kit with 1 g hygromycin and 1 g Zeocin™ at considerable savings. Hygromycin should be stored at +4°C and Zeocin™ at -20°C. Zeocin™ should be protected from exposure to light. All reagents are stable for 6 months when properly stored.
For Use With (Equipment):
__
Quantity:
1 kit
Substrate Type:
__
Type:
__