K1034
CCF2-FA (free acid)
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1034-Each-of-1 | In Stock | ₹ 65,367.84 |
K1034 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 65,367.84
GST (18%): ₹ 11,766.211
Total Price: ₹ 77,134.051
Cell Permeability
Cell-Impermeant
Detection Method
Fluorescence
Product Line
GeneBLAzer™
Substrate
CCF2-FA
Substrate Type
Beta-Lactamase Substrate
Content And Storage
Store in freezer (-5 to -30°C).
Form
Powder
Quantity
100μg
Substrate Properties
Chemical Substrate
Shipping Condition
Approved for shipment on Wet or Dry Ice
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Description
- CCF2-FA (Free Acid) is a fluorescence resonance energy transfer (FRET) substrate that consists of a cephalosporin core linking B7-hydroxycoumarin to fluorescein
- This product is intended for use as a beta-lactamase substrate in a GeneBLAzer™ cell-based assay
- GeneBLAzer™ cell-based assays typically utilize the membrane-permeant ester forms (CCF2-AM and CCF4-AM) of the negatively charged fluorescent beta-lactamase substrates, CCF2 and CCF4
- These lipophilic esters readily enter the cell, where cleavage by endogeneous cytoplasmic esterases rapidly converts them into their negatively charged forms, thereby trapping them in the cytosol
- CCF2-FA is essentially the CCF2 substrate without the esters found in the AM version
- CCF2-FA is de-esterified and used in cell lysate applications, bypassing loading across the cell membrane and de-esterification steps
- Cell lysates are the preferred method for applications using cells that contain a cell wall
- CCF2-FA can also be used as a control to acquire the excitation and emission spectra for CCF2-AM and CCF4-AM.
Compare Similar Items
Show Difference
Cell Permeability: Cell-Impermeant
Detection Method: Fluorescence
Product Line: GeneBLAzer™
Substrate: CCF2-FA
Substrate Type: Beta-Lactamase Substrate
Content And Storage: Store in freezer (-5 to -30°C).
Form: Powder
Quantity: 100μg
Substrate Properties: Chemical Substrate
Shipping Condition: Approved for shipment on Wet or Dry Ice
Cell Permeability:
Cell-Impermeant
Detection Method:
Fluorescence
Product Line:
GeneBLAzer™
Substrate:
CCF2-FA
Substrate Type:
Beta-Lactamase Substrate
Content And Storage:
Store in freezer (-5 to -30°C).
Form:
Powder
Quantity:
100μg
Substrate Properties:
Chemical Substrate
Shipping Condition:
Approved for shipment on Wet or Dry Ice
Cell Permeability: __
Detection Method: Fluorescence
Product Line: GeneBLAzer™
Substrate: __
Substrate Type: __
Content And Storage: GeneBLAzer™ CMV-blaJurkat Positive Control Cell Line is shipped on dry ice and should be stored in liquid nitrogen.
Form: __
Quantity: 1 vial
Substrate Properties: __
Shipping Condition: Dry Ice
Cell Permeability:
__
Detection Method:
Fluorescence
Product Line:
GeneBLAzer™
Substrate:
__
Substrate Type:
__
Content And Storage:
GeneBLAzer™ CMV-blaJurkat Positive Control Cell Line is shipped on dry ice and should be stored in liquid nitrogen.
Form:
__
Quantity:
1 vial
Substrate Properties:
__
Shipping Condition:
Dry Ice
Cell Permeability: __
Detection Method: __
Product Line: GeneSwitch™
Substrate: __
Substrate Type: __
Content And Storage: The GeneSwitch™ System Core Kit includes 20 μg of each of the following vectors: pGene/V5-His (A, B, and C), the expression control vector pGene/V5-His/lacZ, and the pSwitch vector. The kit also includes forward and reverse sequencing primers and 100 μg of the inducing agent mifepristone. The GeneSwitch™ Complete Kit includes the Core Kit with 1 g hygromycin and 1 g Zeocin™ at considerable savings. Hygromycin should be stored at +4°C and Zeocin™ at -20°C. Zeocin™ should be protected from exposure to light. All reagents are stable for 6 months when properly stored.
Form: __
Quantity: 1 kit
Substrate Properties: __
Shipping Condition: __
Cell Permeability:
__
Detection Method:
__
Product Line:
GeneSwitch™
Substrate:
__
Substrate Type:
__
Content And Storage:
The GeneSwitch™ System Core Kit includes 20 μg of each of the following vectors: pGene/V5-His (A, B, and C), the expression control vector pGene/V5-His/lacZ, and the pSwitch vector. The kit also includes forward and reverse sequencing primers and 100 μg of the inducing agent mifepristone. The GeneSwitch™ Complete Kit includes the Core Kit with 1 g hygromycin and 1 g Zeocin™ at considerable savings. Hygromycin should be stored at +4°C and Zeocin™ at -20°C. Zeocin™ should be protected from exposure to light. All reagents are stable for 6 months when properly stored.
Form:
__
Quantity:
1 kit
Substrate Properties:
__
Shipping Condition:
__
Cell Permeability: Cell-Permeant
Detection Method: Fluorescence
Product Line: LiveBLAzer™
Substrate: CCF4-AM
Substrate Type: Beta-Lactamase Substrate
Content And Storage: GeneBLAzer™ beta-lactamase substrates are supplied as loading kits (Table 1) and are available in bulk sizes. Loading kits include CCF2-AM or CCF4-AM, DMSO (for Solution A), Solution B, and Solution C. CCF2-AM and CCF4-AM are provided dry, and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A), Solution B, and Solution C should be stored at 22-25°C, protected from direct light. Slightly cooler temperatures may cause a white precipitate to form in Solution B.
Form: __
Quantity: 200 μg
Substrate Properties: Chemical Substrate
Shipping Condition: __
Cell Permeability:
Cell-Permeant
Detection Method:
Fluorescence
Product Line:
LiveBLAzer™
Substrate:
CCF4-AM
Substrate Type:
Beta-Lactamase Substrate
Content And Storage:
GeneBLAzer™ beta-lactamase substrates are supplied as loading kits (Table 1) and are available in bulk sizes. Loading kits include CCF2-AM or CCF4-AM, DMSO (for Solution A), Solution B, and Solution C. CCF2-AM and CCF4-AM are provided dry, and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A), Solution B, and Solution C should be stored at 22-25°C, protected from direct light. Slightly cooler temperatures may cause a white precipitate to form in Solution B.
Form:
__
Quantity:
200 μg
Substrate Properties:
Chemical Substrate
Shipping Condition:
__