CCF2-FA (free acid)

Description

• CCF2-FA (Free Acid) is a fluorescence resonance energy transfer (FRET) substrate that consists of a cephalosporin core linking B7-hydroxycoumarin to fluorescein
• This product is intended for use as a beta-lactamase substrate in a GeneBLAzer™ cell-based assay
• GeneBLAzer™ cell-based assays typically utilize the membrane-permeant ester forms (CCF2-AM and CCF4-AM) of the negatively charged fluorescent beta-lactamase substrates, CCF2 and CCF4
• These lipophilic esters readily enter the cell, where cleavage by endogeneous cytoplasmic esterases rapidly converts them into their negatively charged forms, thereby trapping them in the cytosol
• CCF2-FA is essentially the CCF2 substrate without the esters found in the AM version
• CCF2-FA is de-esterified and used in cell lysate applications, bypassing loading across the cell membrane and de-esterification steps
• Cell lysates are the preferred method for applications using cells that contain a cell wall
• CCF2-FA can also be used as a control to acquire the excitation and emission spectra for CCF2-AM and CCF4-AM.