K1027

CCF2-FA (free acid)

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 K1027-Each-of-1 In Stock ₹ 4,92,825.60

K1027 - Each of 1

₹ 4,92,825.60

In Stock

Quantity

1

Base Price: ₹ 4,92,825.60

GST (18%): ₹ 88,708.608

Total Price: ₹ 5,81,534.208

Cell Permeability

Cell-Impermeant

Detection Method

Fluorescence

Quantity

1mg

Substrate Properties

Chemical Substrate

Shipping Condition

Approved for shipment on Wet or Dry Ice

Content And Storage

Store in freezer (-5 to -30°C).

Product Line

GeneBLAzer™

Substrate

CCF2-FA

Substrate Type

Beta-Lactamase Substrate

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Description

  • CCF2-FA (Free Acid) is a fluorescence resonance energy transfer (FRET) substrate that consists of a cephalosporin core linking B7-hydroxycoumarin to fluorescein
  • This product is intended for use as a beta-lactamase substrate in a GeneBLAzer™ cell-based assay
  • GeneBLAzer™ cell-based assays typically utilize the membrane-permeant ester forms (CCF2-AM and CCF4-AM) of the negatively charged fluorescent beta-lactamase substrates, CCF2 and CCF4
  • These lipophilic esters readily enter the cell, where cleavage by endogeneous cytoplasmic esterases rapidly converts them into their negatively charged forms, thereby trapping them in the cytosol
  • CCF2-FA is essentially the CCF2 substrate without the esters found in the AM version
  • CCF2-FA is de-esterified and used in cell lysate applications, bypassing loading across the cell membrane and de-esterification steps
  • Cell lysates are the preferred method for applications using cells that contain a cell wall
  • CCF2-FA can also be used as a control to acquire the excitation and emission spectra for CCF2-AM and CCF4-AM.

Compare Similar Items

Show Difference

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Fischer Scientific

K1027

--


Cell Permeability:
Cell-Impermeant

Detection Method:
Fluorescence

Quantity:
1mg

Substrate Properties:
Chemical Substrate

Shipping Condition:
Approved for shipment on Wet or Dry Ice

Content And Storage:
Store in freezer (-5 to -30°C).

Product Line:
GeneBLAzer™

Substrate:
CCF2-FA

Substrate Type:
Beta-Lactamase Substrate

Img

Fischer Scientific

K1028

--


Cell Permeability:
Cell-Permeant

Detection Method:
Fluorescence

Quantity:
5mg

Substrate Properties:
Chemical Substrate

Shipping Condition:
Room Temperature

Content And Storage:
Store in freezer (-5 to -30°C).

Product Line:
GeneBLAzer™

Substrate:
CCF4-AM

Substrate Type:
Beta-Lactamase Substrate

Img

Fischer Scientific

K1029

--


Cell Permeability:
Cell-Permeant

Detection Method:
Fluorescence

Quantity:
20mg

Substrate Properties:
Chemical Substrate

Shipping Condition:
Room Temperature

Content And Storage:
Store in freezer (-5 to -30°C).

Product Line:
LiveBLAzer™

Substrate:
CCF4-AM

Substrate Type:
Beta-Lactamase Substrate

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Fischer Scientific

K1030

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Cell Permeability:
Cell-Permeant

Detection Method:
Fluorescence

Quantity:
5 mg

Substrate Properties:
Chemical Substrate

Shipping Condition:
__

Content And Storage:
GeneBLAzer™ beta-lactamase substrates are supplied as loading kits (Table 1) and are available in bulk sizes. Loading kits include CCF2-AM or CCF4-AM, DMSO (for Solution A), Solution B, and Solution C. CCF2-AM and CCF4-AM are provided dry, and should be stored at -20°C, desiccated and protected from light. DMSO (for Solution A), Solution B, and Solution C should be stored at 22-25°C, protected from direct light. Slightly cooler temperatures may cause a white precipitate to form in Solution B.

Product Line:
LiveBLAzer™

Substrate:
CCF4-AM

Substrate Type:
Beta-Lactamase Substrate