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K1329

Thermo Scientific™ GeneBLAzer™ AVPR2 CHO-K1 DA Assay Kit

Manufacturer: Thermo Scientific™

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Pack Size	SKU	Availability	Price
Each of 1	K1329-Each-of-1	In Stock	₹ 98,736.24

K1329 - Each of 1

₹ 98,736.24

In Stock

Quantity

Base Price: ₹ 98,736.24

GST (18%): ₹ 17,772.523

Total Price: ₹ 1,16,508.763

Accession Number

NM_000054

Cell State

Division-Arrested Cells

Gene ID (Entrez)

554

Population Status

Single Cell Clone Origin

Quantity

1 plate

Target Entry

AVPR2

System Type

GeneBLAzer™

Assay Entry

Cell-based beta lactamase reporter gene

Detection Method

Fluorescence

Gene Symbol

AVPR2

Product Line

GeneBLAzer™

Reporter Gene

BLA (Beta-Lactamase)

Cell Line

CHO-K1

Druggable Target

GPCR

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Description

CellSensor™ CRE- bla CHO-K1 cells (Cat
no.K1129) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE)
Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate) or a tube of cells sufficient to analyze 10 x 384-well plates
DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
Both AVPR2 CHO-K1 DA cells and AVPR2-CRE- bla CHO-K1 cells are functionally validated for Z’-factor and EC 50 concentrations of dDAVP (Figure 1)
In addition, AVPR2-CRE- bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request)
Additional testing data using alternate stimuli are also available
Agonists and antagonists of the AVPR2 receptors are in various stages of clinical trials for the treatment of hypertension and as diuretics
Storage: GeneBLAzer™ AVPR2 CHO-K1 DA Cells and AVPR2-CRE- bla CHO-K1 Cells are shipped on dry ice
Store in liquid nitrogen immediately upon receipt or thaw for immediate use
Contents: GeneBLAzer™ AVPR2 CHO-K1 DA Assay Kit Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate
(Other materials are required separately; please refer to the protocol)
Includes: •AVPR2 CHO-K1 DA cells •LiveBLAzer™-FRET B/G Loading Kit, 70μg •Solution D, 1ml •2 protocols •Certificate of Analysis GeneBLAzer™ AVPR2 CHO-K1 DA cells Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol)
Includes: •AVPR2 CHO-K1 DA cells •1 protocol •Certificate of Analysis GeneBLAzer™ AVPR2-CRE- bla CHO-K1 cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol)
Includes: •AVPR2-CRE- bla CHO-K1 cells •1 protocol •Certificate of Analysis

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