K1875
Thermo Scientific™ GeneBLAzer™ PPAR alpha UAS-blaHEK 293T Cellular Assay Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1875-Each-of-1 | In Stock | ₹ 1,29,366.72 |
K1875 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,29,366.72
GST (18%): ₹ 23,286.01
Total Price: ₹ 1,52,652.73
Accession Number
NM_005036
Cell State
Transiently Transduced
Detection Method
Fluorescence
Gene Symbol
PPARA
Product Line
GeneBLAzer™
Readout
End Point
Target Entry
PPARA, PPAR alpha, NR1C1
Cell Line
HEK 293T
Druggable Target
Nuclear Receptors
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
• PPAR alpha UAS-bla HEK293T cells (K1875A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
For Use With (Application)
FRET
Human Protein Class
NHR
Quantity
1 Plate
Reporter Gene
BLA (Beta-Lactamase)
Type
Cellular Assay Kit
System Type
GeneBLAzer™
Related Products
Description
- The GeneBLAzer™ PPAR alpha-UAS-bla HEK 293T Cellular Assay provides an accurate, sensitive, and easy-to-use method for monitoring the cellular response of PPAR alpha to drug candidates or other stimuli
- Features of the The GeneBLAzer™ PPAR alpha-UAS-bla HEK 293T Cellular Assay: Convenience —plate cells and perform assay without additional cell culture steps Low Noise —the kit uses a Fluorescence Resonance Energy Transfer (FRET)-based ratiometric readout for minimum noise and error Low Background —the GAL4 fusion prevents any background from endogenous receptor The Cells The GeneBLAzer™ PPAR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human peroxisome proliferator-activated receptor alpha (PPAR alpha) fused to the DNA-binding domain of GAL4 transiently transduced (via BacMam virus) into the GeneBLAzer™ UAS-bla HEK293T cell line (Cat
- No
- K1711)
- GeneBLAzer™ UAS-bla HEK 293T cells stably express a β-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS)
- The Assay When an agonist binds to the LBD of the GAL4 (DBD)-PPAR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of β-lactamase
- The FRET ratiometric readout from the live-cell substrate reduces the absolute and relative errors that can mask the underlying biological response of interest
- PPAR alpha UAS-bla HEK 293T cells have been tested for assay performance using variable assay conditions, including cell number, stimulation time, substrate loading time and have been validated for Z' and EC 50 concentrations of GW7647
- Additional testing data using alternate stimuli are also available
- See the 'validation packet' in the documents below to view the data
- Speed and Convenience Because PPAR alpha UAS-bla HEK 293T cells are transiently transduced with BacMam virus, they are ready to be used in an assay without any additional cell culture steps
- Just plate the cells and assay as outlined in the protocol
- Please contact discoveryservices@lifetech.com to inquire about obtaining these cells at economical bulk scales.
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Accession Number: NM_005036
Cell State: Transiently Transduced
Detection Method: Fluorescence
Gene Symbol: PPARA
Product Line: GeneBLAzer™
Readout: End Point
Target Entry: PPARA, PPAR alpha, NR1C1
Cell Line: HEK 293T
Druggable Target: Nuclear Receptors
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: • PPAR alpha UAS-bla HEK293T cells (K1875A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
For Use With (Application): FRET
Human Protein Class: NHR
Quantity: 1 Plate
Reporter Gene: BLA (Beta-Lactamase)
Type: Cellular Assay Kit
System Type: GeneBLAzer™
Accession Number:
NM_005036
Cell State:
Transiently Transduced
Detection Method:
Fluorescence
Gene Symbol:
PPARA
Product Line:
GeneBLAzer™
Readout:
End Point
Target Entry:
PPARA, PPAR alpha, NR1C1
Cell Line:
HEK 293T
Druggable Target:
Nuclear Receptors
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
• PPAR alpha UAS-bla HEK293T cells (K1875A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
For Use With (Application):
FRET
Human Protein Class:
NHR
Quantity:
1 Plate
Reporter Gene:
BLA (Beta-Lactamase)
Type:
Cellular Assay Kit
System Type:
GeneBLAzer™
Accession Number: __
Cell State: __
Detection Method: __
Gene Symbol: __
Product Line: __
Readout: __
Target Entry: __
Cell Line: __
Druggable Target: __
Assay Entry: __
Content And Storage: __
For Use With (Application): __
Human Protein Class: __
Quantity: __
Reporter Gene: __
Type: __
System Type: __
Accession Number:
__
Cell State:
__
Detection Method:
__
Gene Symbol:
__
Product Line:
__
Readout:
__
Target Entry:
__
Cell Line:
__
Druggable Target:
__
Assay Entry:
__
Content And Storage:
__
For Use With (Application):
__
Human Protein Class:
__
Quantity:
__
Reporter Gene:
__
Type:
__
System Type:
__
Accession Number: __
Cell State: __
Detection Method: __
Gene Symbol: __
Product Line: __
Readout: __
Target Entry: __
Cell Line: __
Druggable Target: __
Assay Entry: __
Content And Storage: __
For Use With (Application): __
Human Protein Class: __
Quantity: __
Reporter Gene: __
Type: __
System Type: __
Accession Number:
__
Cell State:
__
Detection Method:
__
Gene Symbol:
__
Product Line:
__
Readout:
__
Target Entry:
__
Cell Line:
__
Druggable Target:
__
Assay Entry:
__
Content And Storage:
__
For Use With (Application):
__
Human Protein Class:
__
Quantity:
__
Reporter Gene:
__
Type:
__
System Type:
__
Accession Number: NM_000868.1
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: __
Product Line: GeneBLAzer™
Readout: End Point
Target Entry: HTR2C
Cell Line: CHO-K1
Druggable Target: GPCR
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: GeneBLAzer™ cell lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application): FRET
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Quantity: 8 x 106Cells
Reporter Gene: BLA (Beta-Lactamase)
Type: __
System Type: GeneBLAzer™
Accession Number:
NM_000868.1
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
__
Product Line:
GeneBLAzer™
Readout:
End Point
Target Entry:
HTR2C
Cell Line:
CHO-K1
Druggable Target:
GPCR
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
GeneBLAzer™ cell lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application):
FRET
Human Protein Class:
G-Protein Coupled Receptor (GPCR)
Quantity:
8 x 106Cells
Reporter Gene:
BLA (Beta-Lactamase)
Type:
__
System Type:
GeneBLAzer™