K1395
Thermo Scientific™ GeneBLAzer™ PPAR delta DA Assay Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1395-Each-of-1 | In Stock | ₹ 1,31,762.40 |
K1395 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,31,762.40
GST (18%): ₹ 23,717.232
Total Price: ₹ 1,55,479.632
Alternate Agonists
Estrone, Genistein, Diethylstibestrol, Ethynylestradiol
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:• PPAR delta HEK 293T DA cells (K1395A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene ID (Entrez)
5467
Primary Agonist
17-β-estradiol
Quantity
1 plate
Target Entry
PPAR delta, NR1C2, PPARD
Cell Line
293T (HEK)
Druggable Target
Nuclear Receptors
Antagonists
4-OH-tam+0.2 nM E2, Tam+0.2 nM E2, 4-OH-tamoxifen, Tamoxifen, RU486+0.2 nM E2, RU486
Cell State
Division-Arrested Cells
Detection Method
Fluorescence
Gene Symbol
PPARδ
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Type
Division-Arrested Cells Assay Kit
System Type
GeneBLAzer™
Related Products
Description
- GeneBLAzer™ PPAR delta DA (Division Arrested) cells and PPAR delta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-delta (PPAR delta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla HEK 293T cell line
- GeneBLAzer™ UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS)
- When an agonist binds to the LBD of the GAL4 (DBD)-PPAR delta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase
- Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
- Both PPAR delta DA cells and PPAR delta-UAS-bla HEK 293T cells are functionally validated for Z' and EC 50 concentrations of L-165041
- In addition, PPAR delta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request)
- Additional testing data using alternate stimuli are also available.
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Alternate Agonists: Estrone, Genistein, Diethylstibestrol, Ethynylestradiol
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:• PPAR delta HEK 293T DA cells (K1395A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene ID (Entrez): 5467
Primary Agonist: 17-β-estradiol
Quantity: 1 plate
Target Entry: PPAR delta, NR1C2, PPARD
Cell Line: 293T (HEK)
Druggable Target: Nuclear Receptors
Antagonists: 4-OH-tam+0.2 nM E2, Tam+0.2 nM E2, 4-OH-tamoxifen, Tamoxifen, RU486+0.2 nM E2, RU486
Cell State: Division-Arrested Cells
Detection Method: Fluorescence
Gene Symbol: PPARδ
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Type: Division-Arrested Cells Assay Kit
System Type: GeneBLAzer™
Alternate Agonists:
Estrone, Genistein, Diethylstibestrol, Ethynylestradiol
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:• PPAR delta HEK 293T DA cells (K1395A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene ID (Entrez):
5467
Primary Agonist:
17-β-estradiol
Quantity:
1 plate
Target Entry:
PPAR delta, NR1C2, PPARD
Cell Line:
293T (HEK)
Druggable Target:
Nuclear Receptors
Antagonists:
4-OH-tam+0.2 nM E2, Tam+0.2 nM E2, 4-OH-tamoxifen, Tamoxifen, RU486+0.2 nM E2, RU486
Cell State:
Division-Arrested Cells
Detection Method:
Fluorescence
Gene Symbol:
PPARδ
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Type:
Division-Arrested Cells Assay Kit
System Type:
GeneBLAzer™
Alternate Agonists: Cholic Acid, Deoxycholic Acid
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ER beta HEK 293 T DA cells (K1399A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
Gene ID (Entrez): 2100
Primary Agonist: Chenodeoxycholic Acid (CDCA)
Quantity: 1 plate
Target Entry: ER beta, ESR2, ERB, NR3A2
Cell Line: 293T (HEK)
Druggable Target: Nuclear Receptors
Antagonists: (z)-guggulsterone
Cell State: Division-Arrested Cells
Detection Method: Fluorescence
Gene Symbol: ERβ
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Type: __
System Type: GeneBLAzer™
Alternate Agonists:
Cholic Acid, Deoxycholic Acid
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ER beta HEK 293 T DA cells (K1399A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
Gene ID (Entrez):
2100
Primary Agonist:
Chenodeoxycholic Acid (CDCA)
Quantity:
1 plate
Target Entry:
ER beta, ESR2, ERB, NR3A2
Cell Line:
293T (HEK)
Druggable Target:
Nuclear Receptors
Antagonists:
(z)-guggulsterone
Cell State:
Division-Arrested Cells
Detection Method:
Fluorescence
Gene Symbol:
ERβ
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Type:
__
System Type:
GeneBLAzer™
Alternate Agonists: Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:• RAR beta HEK 293T DA cells (K1407A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene ID (Entrez): __
Primary Agonist: 9-cis-retinoic acid
Quantity: 1 plate
Target Entry: RAR beta, NR1B2, RARB
Cell Line: 293T (HEK)
Druggable Target: Nuclear Receptors
Antagonists: none
Cell State: Division-Arrested Cells
Detection Method: Fluorescence
Gene Symbol: RARβ
Product Line: __
Reporter Gene: BLA (Beta-Lactamase)
Type: Division-Arrested Cells Assay Kit
System Type: GeneBLAzer™
Alternate Agonists:
Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:• RAR beta HEK 293T DA cells (K1407A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene ID (Entrez):
__
Primary Agonist:
9-cis-retinoic acid
Quantity:
1 plate
Target Entry:
RAR beta, NR1B2, RARB
Cell Line:
293T (HEK)
Druggable Target:
Nuclear Receptors
Antagonists:
none
Cell State:
Division-Arrested Cells
Detection Method:
Fluorescence
Gene Symbol:
RARβ
Product Line:
__
Reporter Gene:
BLA (Beta-Lactamase)
Type:
Division-Arrested Cells Assay Kit
System Type:
GeneBLAzer™
Alternate Agonists: L165, 041, GW 1929, GW 7647, GW 0742
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ERR alpha HEK 293 T DA cells (K1421A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
Gene ID (Entrez): 2101
Primary Agonist: Rosiglitazone
Quantity: 1 plate
Target Entry: ESRRA, NR3B1, ERR1, ERR alpha
Cell Line: 293T (HEK)
Druggable Target: Nuclear Receptors
Antagonists: T0070907, GW 9662, GW 6471
Cell State: Division-Arrested Cells
Detection Method: Fluorescence
Gene Symbol: ERRα
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Type: __
System Type: GeneBLAzer™
Alternate Agonists:
L165, 041, GW 1929, GW 7647, GW 0742
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ERR alpha HEK 293 T DA cells (K1421A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
Gene ID (Entrez):
2101
Primary Agonist:
Rosiglitazone
Quantity:
1 plate
Target Entry:
ESRRA, NR3B1, ERR1, ERR alpha
Cell Line:
293T (HEK)
Druggable Target:
Nuclear Receptors
Antagonists:
T0070907, GW 9662, GW 6471
Cell State:
Division-Arrested Cells
Detection Method:
Fluorescence
Gene Symbol:
ERRα
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Type:
__
System Type:
GeneBLAzer™