K1559
Thermo Scientific™ GeneBLAzer™ RAR-gamma DA Assay Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1559-Each-of-1 | In Stock | ₹ 1,30,222.32 |
K1559 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,30,222.32
GST (18%): ₹ 23,440.018
Total Price: ₹ 1,53,662.338
Alternate Agonists
4-hydroxytamoxifen, Chlordane, Toxaphene
Cell State
Division-Arrested Cells
Detection Method
Fluorescence
Primary Agonist
XCT790
Reporter Gene
BLA (Beta-Lactamase)
Type
Division-Arrested Cells Assay Kit
System Type
GeneBLAzer™
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate.• RAR gamma HEK 293T DA cells (K1559A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene Symbol
RARγ
Quantity
1 Plate
Target Entry
RARG, RAR gamma, NR1B3
Cell Line
293T (HEK)
Druggable Target
Nuclear Receptors
Related Products
Description
- GeneBLAzer™RAR gamma DA (Division Arrested) cells and RAR gamma-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor gamma (RAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line
- GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS)
- When an agonist binds to the LBD of the GAL4 (DBD)-RAR gamma (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
- Both RAR gamma DA cells and RAR gamma-UAS-bla HEK 293T cells are functionally validated for Z' and EC 50 concentrations of all-trans retinoic acid (ATRA)
- In addition, RAR gamma-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
Compare Similar Items
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Alternate Agonists: 4-hydroxytamoxifen, Chlordane, Toxaphene
Cell State: Division-Arrested Cells
Detection Method: Fluorescence
Primary Agonist: XCT790
Reporter Gene: BLA (Beta-Lactamase)
Type: Division-Arrested Cells Assay Kit
System Type: GeneBLAzer™
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate.• RAR gamma HEK 293T DA cells (K1559A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene Symbol: RARγ
Quantity: 1 Plate
Target Entry: RARG, RAR gamma, NR1B3
Cell Line: 293T (HEK)
Druggable Target: Nuclear Receptors
Alternate Agonists:
4-hydroxytamoxifen, Chlordane, Toxaphene
Cell State:
Division-Arrested Cells
Detection Method:
Fluorescence
Primary Agonist:
XCT790
Reporter Gene:
BLA (Beta-Lactamase)
Type:
Division-Arrested Cells Assay Kit
System Type:
GeneBLAzer™
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate.• RAR gamma HEK 293T DA cells (K1559A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene Symbol:
RARγ
Quantity:
1 Plate
Target Entry:
RARG, RAR gamma, NR1B3
Cell Line:
293T (HEK)
Druggable Target:
Nuclear Receptors
Alternate Agonists: __
Cell State: __
Detection Method: __
Primary Agonist: __
Reporter Gene: __
Type: __
System Type: __
Assay Entry: __
Content And Storage: • 17.5 mL Lysis Buffer L3; store at room temperature• 175 mL Lysis Buffer L5; room temperature• 60 mL Wash Buffer W4; room temperature• 10 mL Wash Buffer W5; room temperature• 5 mL RNase-free Water; room temperature• 50 Spin Cartridges with Collection Tubes; room temperature• 50 Wash Tubes (2 mL); room temperature• 50 Elution Tubes (1.7 mL); room temperature
Gene Symbol: __
Quantity: 50 preps
Target Entry: __
Cell Line: __
Druggable Target: __
Alternate Agonists:
__
Cell State:
__
Detection Method:
__
Primary Agonist:
__
Reporter Gene:
__
Type:
__
System Type:
__
Assay Entry:
__
Content And Storage:
• 17.5 mL Lysis Buffer L3; store at room temperature• 175 mL Lysis Buffer L5; room temperature• 60 mL Wash Buffer W4; room temperature• 10 mL Wash Buffer W5; room temperature• 5 mL RNase-free Water; room temperature• 50 Spin Cartridges with Collection Tubes; room temperature• 50 Wash Tubes (2 mL); room temperature• 50 Elution Tubes (1.7 mL); room temperature
Gene Symbol:
__
Quantity:
50 preps
Target Entry:
__
Cell Line:
__
Druggable Target:
__
Alternate Agonists: __
Cell State: __
Detection Method: __
Primary Agonist: __
Reporter Gene: __
Type: __
System Type: __
Assay Entry: __
Content And Storage: • 15 mL Melting Buffer; store at room temperature• 1 mL Proteinase K in buffer; room temperature• 20 mL Binding Buffer L3; room temperature• 20 mL Wash Buffer W5; room temperature• 4 mL RNase-free Water; room temperature• 50 Spin Cartridges with Collection Tubes; room temperature• 50 Wash Tubes; room temperature• 50 Recovery Tubes; room temperature• 0.1 mL DNase I, Amplification Grade; -20°C
Gene Symbol: __
Quantity: 50 Preps
Target Entry: __
Cell Line: __
Druggable Target: __
Alternate Agonists:
__
Cell State:
__
Detection Method:
__
Primary Agonist:
__
Reporter Gene:
__
Type:
__
System Type:
__
Assay Entry:
__
Content And Storage:
• 15 mL Melting Buffer; store at room temperature• 1 mL Proteinase K in buffer; room temperature• 20 mL Binding Buffer L3; room temperature• 20 mL Wash Buffer W5; room temperature• 4 mL RNase-free Water; room temperature• 50 Spin Cartridges with Collection Tubes; room temperature• 50 Wash Tubes; room temperature• 50 Recovery Tubes; room temperature• 0.1 mL DNase I, Amplification Grade; -20°C
Gene Symbol:
__
Quantity:
50 Preps
Target Entry:
__
Cell Line:
__
Druggable Target:
__
Alternate Agonists: __
Cell State: Dividing Cells
Detection Method: Fluorescence
Primary Agonist: __
Reporter Gene: BLA (Beta-Lactamase)
Type: __
System Type: GeneBLAzer™
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ GRPR-NFAT-bla CHO-K1 cells• GeneBLAzer™ GRPR-NFAT-bla CHO-K1 Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ GRPR-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Gene Symbol: GRPR
Quantity: 2 x 106Cells
Target Entry: GRPR
Cell Line: CHO-K1
Druggable Target: GPCR
Alternate Agonists:
__
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Primary Agonist:
__
Reporter Gene:
BLA (Beta-Lactamase)
Type:
__
System Type:
GeneBLAzer™
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ GRPR-NFAT-bla CHO-K1 cells• GeneBLAzer™ GRPR-NFAT-bla CHO-K1 Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ GRPR-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Gene Symbol:
GRPR
Quantity:
2 x 106Cells
Target Entry:
GRPR
Cell Line:
CHO-K1
Druggable Target:
GPCR